Plasmodium falciparum Malaria: reduction of endothelial cell apoptosis in vitro

Details

Serval ID
serval:BIB_743DDFD71478
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Plasmodium falciparum Malaria: reduction of endothelial cell apoptosis in vitro
Journal
Infection and Immunity
Author(s)
Hemmer  C. J., Lehr  H. A., Westphal  K., Unverricht  M., Kratzius  M., Reisinger  E. C.
ISSN
0019-9567 (Print)
Publication state
Published
Issued date
2005
Volume
73
Number
3
Pages
1764-1770
Notes
DA - 20050225LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov'tRN - 0 (Antioxidants)RN - 0 (Immune Sera)RN - 0 (Protease Inhibitors)SB - IM
Abstract
Organ failure in Plasmodium falciparum malaria is associated with neutrophil activation and endothelial damage. This study investigates whether neutrophil-induced endothelial damage involves apoptosis and whether it can be prevented by neutralization of neutrophil secretory products. Endothelial cells from human umbilical veins were coincubated with neutrophils from healthy donors and with sera from eight patients with P. falciparum malaria, three patients with P. vivax malaria, and three healthy controls. Endothelial apoptosis was demonstrated by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) and annexin V staining. The rate of apoptosis of cells was markedly increased after incubation with patient serum compared to that with control serum. Apoptosis was most pronounced after incubation with sera from two patients with fatal cases of P. falciparum malaria, followed by sera of survivors with severe P. falciparum malaria and, finally, by sera of patients with mild P. falciparum and P. vivax malaria. Ascorbic acid, tocopherol, and ulinastatin reduced the apoptosis rate, but gabexate mesilate and pentoxifylline did not. Furthermore, in fatal P. falciparum malaria, apoptotic endothelial cells were identified in renal and pulmonary tissue by TUNEL staining. These findings show that apoptosis caused by neutrophil secretory products plays a major role in endothelial cell damage in malaria. The antioxidants ascorbic acid and tocopherol and the protease inhibitor ulinastatin can reduce malaria-associated endothelial apoptosis in vitro
Keywords
Animals/Antioxidants/pharmacology/Apoptosis/drug effects/Cells,Cultured/Endothelial Cells/physiology/Endothelium,Vascular/cytology/Humans/Immune Sera/immunology/Malaria,Falciparum/mortality/parasitology/physiopathology/Neutrophils/metabolism/Plasmodium falciparum/pathogenicity/Protease Inhibitors/Umbilical Veins
Pubmed
Web of science
Create date
29/01/2008 18:34
Last modification date
20/08/2019 14:32
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