Long-term renewal of hair follicles from clonogenic multipotent stem cells
Details
Serval ID
serval:BIB_7417B399CDD6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Long-term renewal of hair follicles from clonogenic multipotent stem cells
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
10/2005
Volume
102
Number
41
Pages
14677-82
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 11
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 11
Abstract
Adult stem cells are essential for tissue renewal, regeneration, and repair, and their expansion in culture is of paramount importance for regenerative medicine. Using the whisker follicle of the rat as a model system, we demonstrate that (i) clonogenicity is an intrinsic property of the adult stem cells of the hair follicle; (ii) after cultivation for >140 doublings, these stem cells, transplanted to the dermo-epidermal junction of newborn mouse skin, form part or all of the developing follicles; (iii) the stem cells incorporated into follicles are multipotent, because they generate all of the lineages of the hair follicle and sebaceous gland; (iv) thousands of hair follicles can be generated from the progeny of a single cultivated stem cell; (v) cultured stem cells express the self-renewal genes Bmi1 and Zfp145;(vi) several stem cells participate in the formation of a single hair bulb; and (vii) there are many more stem cells in whisker follicles than could be anticipated from label-retaining experiments.
Keywords
Animals
DNA Primers
Hair Follicle/cytology/*physiology
In Situ Hybridization, Fluorescence
Multipotent Stem Cells/*cytology/*metabolism
Nuclear Proteins/metabolism
Proto-Oncogene Proteins/metabolism
Rats
Rats, Inbred F344
Regeneration/*physiology
Repressor Proteins/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stem Cell Transplantation/methods
Zinc Fingers/genetics
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 9:41
Last modification date
20/08/2019 15:31