Increased mobility of major histocompatibility complex I-peptide complexes decreases the sensitivity of antigen recognition.

Details

Serval ID
serval:BIB_73CE09F38E90
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Increased mobility of major histocompatibility complex I-peptide complexes decreases the sensitivity of antigen recognition.
Journal
Journal of Biological Chemistry
Author(s)
Segura J.M., Guillaume P., Mark S., Dojcinovic D., Johannsen A., Bosshard G., Angelov G., Legler D.F., Vogel H., Luescher I.F.
ISSN
0021-9258
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
283
Number
35
Pages
24254-24263
Language
english
Abstract
CD8(+) cytotoxic T lymphocytes (CTL) can recognize and kill target cells expressing only a few cognate major histocompatibility complex (MHC) I-peptide complexes. This high sensitivity requires efficient scanning of a vast number of highly diverse MHC I-peptide complexes by the T cell receptor in the contact site of transient conjugates formed mainly by nonspecific interactions of ICAM-1 and LFA-1. Tracking of single H-2K(d) molecules loaded with fluorescent peptides on target cells and nascent conjugates with CTL showed dynamic transitions between states of free diffusion and immobility. The immobilizations were explained by association of MHC I-peptide complexes with ICAM-1 and strongly increased their local concentration in cell adhesion sites and hence their scanning by T cell receptor. In nascent immunological synapses cognate complexes became immobile, whereas noncognate ones diffused out again. Interfering with this mobility modulation-based concentration and sorting of MHC I-peptide complexes strongly impaired the sensitivity of antigen recognition by CTL, demonstrating that it constitutes a new basic aspect of antigen presentation by MHC I molecules.
Keywords
Animals, Antigen Presentation, CD8-Positive T-Lymphocytes/immunology, Cell Adhesion/genetics, Cell Adhesion/immunology, Cell Movement/genetics, Cell Movement/immunology, H-2 Antigens/genetics, H-2 Antigens/immunology, Humans, Intercellular Adhesion Molecule-1/genetics, Intercellular Adhesion Molecule-1/immunology, L Cells (Cell Line), Mice, Peptides/genetics, Peptides/immunology, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell/immunology
Pubmed
Web of science
Open Access
Yes
Create date
12/10/2009 11:59
Last modification date
20/08/2019 14:31
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