Physiological and transcriptome changes induced by Pseudomonas putida acquisition of an integrative and conjugative element.

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Version: Final published version
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Serval ID
serval:BIB_73BE1A6DF8A9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Physiological and transcriptome changes induced by Pseudomonas putida acquisition of an integrative and conjugative element.
Journal
Scientific reports
Author(s)
Miyazaki R., Yano H., Sentchilo V., van der Meer J.R.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
03/04/2018
Peer-reviewed
Oui
Volume
8
Number
1
Pages
5550
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Integrative and conjugative elements (ICEs) comprise ubiquitous large mobile regions in prokaryotic chromosomes that transmit vertically to daughter cells and transfer horizontally to distantly related lineages. Their evolutionary success originates in maximized combined ICE-host fitness trade-offs, but how the ICE impacts on the host metabolism and physiology is poorly understood. Here we investigate global changes in the host genetic network and physiology of Pseudomonas putida with or without an integrated ICEclc, a model ICE widely distributed in proteobacterial genomes. Genome-wide gene expression differences were analyzed by RNA-seq using exponentially growing or stationary phase-restimulated cultures on 3-chlorobenzoate, an aromatic compound metabolizable thanks to specific ICEclc-located genes. We found that the presence of ICEclc imposes a variety of changes in global pathways such as cell cycle and amino acid metabolism, which were more numerous in stationary-restimulated than exponential phase cells. Unexpectedly, ICEclc stimulates cellular motility and leads to more rapid growth on 3-chlorobenzoate than cells carrying only the integrated clc genes. ICEclc also concomitantly activates the P. putida Pspu28-prophage, but this in itself did not provoke measurable fitness effects. ICEclc thus interferes in a number of cellular pathways, inducing both direct benefits as well as indirect costs in P. putida.
Keywords
Bacterial Proteins/genetics, Conjugation, Genetic, Gene Expression Regulation, Bacterial, Genome, Bacterial, Genomic Islands, Interspersed Repetitive Sequences, Prophages/genetics, Pseudomonas putida/genetics, Pseudomonas putida/physiology, Transcriptome
Pubmed
Web of science
Open Access
Yes
Create date
12/04/2018 17:33
Last modification date
26/10/2019 6:09
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