Differential phosphorylation of MAP1b during postnatal development of the cat brain.

Details

Serval ID
serval:BIB_73454690827A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential phosphorylation of MAP1b during postnatal development of the cat brain.
Journal
Journal of Neurocytology
Author(s)
Riederer B.M.
ISSN
0300-4864 (Print)
ISSN-L
0300-4864
Publication state
Published
Issued date
1995
Volume
24
Number
1
Pages
45-54
Language
english
Abstract
Microtubule-associated protein 1b, previously also referred to as microtubule-associated protein 5 or microtubule-associated protein 1x, is a major component of the juvenile cytoskeleton, and is essential during the early differentiation of neurons. It is required for axonal growth and its function is influenced by phosphorylation. The distribution of microtubule-associated protein 1b in kitten cerebellum and cortex during postnatal development was studied with two monoclonal antibodies. Hybridoma clone AA6 detected a non-phosphorylated site, while clone 125 detected a site phosphorylated by casein-kinase II. On blots, both monoclonal antibodies stained the same two proteins of similar molecular weights, also referred to as microtubule-associated protein 5a and 5b. Antibody 125 detected a phosphorylated epitope on both microtubule-associated protein 1b forms; dephosphorylation by alkaline phosphatase abolished the immunological detection. During development of cat cortex and cerebellum, AA6 stained the perikarya and dendrites of neurons during their early differentiation, and especially labelled newly generated axons. The staining decreased during development, and axonal staining was reduced in adult tissue. In contrast to previous reports which demonstrated that antibodies against phosphorylated microtubule-associated protein 1b label exclusively axons, antibody 125 also localized microtubule-associated protein 1b in cell bodies and dendrites, even in adulthood. Some nuclear staining was observed, indicating that a phosphorylated form of microtubule-associated protein 1b may participate in nuclear function. These results demonstrate that microtubule-associated protein 1b is subject to CK2-type phosphorylation throughout neuronal maturation and suggest that phosphorylation of microtubule-associated protein 1b may participate in juvenile and mature-type microtubule functions throughout development.
Keywords
Animals, Axons/physiology, Brain/growth & development, Brain/metabolism, Cats, Dendrites/physiology, Immunohistochemistry, Microtubule-Associated Proteins/metabolism, Nerve Tissue Proteins/metabolism, Phosphorylation
Pubmed
Web of science
Create date
24/01/2008 14:34
Last modification date
20/08/2019 14:31
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