An ancient truncated duplication of the anti-Mullerian hormone receptor type 2 gene is a potential conserved master sex determinant in the Pangasiidae catfish family.

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Version: author
License: CC BY-NC 4.0
Serval ID
serval:BIB_72F2B4092924
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An ancient truncated duplication of the anti-Mullerian hormone receptor type 2 gene is a potential conserved master sex determinant in the Pangasiidae catfish family.
Journal
Molecular ecology resources
Author(s)
Wen M., Pan Q., Jouanno E., Montfort J., Zahm M., Cabau C., Klopp C., Iampietro C., Roques C., Bouchez O., Castinel A., Donnadieu C., Parrinello H., Poncet C., Belmonte E., Gautier V., Avarre J.C., Dugue R., Gustiano R.,  TTT, Campet M., Sriphairoj K., Ribolli J., de Almeida F.L., Desvignes T., Postlethwait J.H., Floi Bucao C., Robinson-Rechavi M., Bobe J., Herpin A., Guiguen Y.
ISSN
1755-0998 (Electronic)
ISSN-L
1755-098X
Publication state
Published
Issued date
2022
Peer-reviewed
Oui
Volume
22
Number
6
Pages
2411-2428
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Abstract
Evolution of sex determination (SD) in teleosts is amazingly dynamic, as reflected by the variety of different master sex-determining genes identified. Pangasiids are economically important catfishes in South-Asian countries, but little is known about their SD system. Here, we generated novel genomic resources for 12 Pangasiids and characterized their SD system. Based on a Pangasianodon hypophthalmus, chromosome-scale genome assembly we identified an anti-Müllerian hormone receptor type Ⅱ gene (amhr2) duplication, which was further characterized as being sex-linked in males and expressed only in testes. These results point to a Y chromosome male-specific duplication (amhr2by) of the autosomal amhr2a. Sequence annotation revealed that the P. hypophthalmus Amhr2by is truncated in its N-terminal domain, lacking the cysteine-rich extracellular part of the receptor that is crucial for ligand binding, suggesting a potential route for its neofunctionalization. Reference-guided assembly of 11 additional Pangasiids, along with sex-linkage studies, revealed that this truncated amhr2by duplication is a male-specific conserved gene in Pangasiids. Reconstructions of the amhr2 phylogeny suggested that amhr2by arose from an ancient duplication / insertion event at the root of the Siluroidei radiation that is dated around 100 million years ago. Altogether these results bring multiple lines of evidence supporting that amhr2by is an ancient and conserved master sex-determining gene in Pangasiids, a finding that highlights the recurrent usage of the transforming growth factor β pathway, which is often used for the recruitment of teleost master sex determination genes, and brings another empirical case towards the understanding of the dynamics of sex determination systems.
Keywords
Genetics, Ecology, Evolution, Behavior and Systematics, Biotechnology, amhr2, Pangasiid catfishes, evolution, male genome assembly, sex determination
Pubmed
Create date
22/04/2022 12:32
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05/07/2022 7:10
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