A morphometric study of mechanotransductively induced dermal neovascularization.

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Version: author
Serval ID
serval:BIB_72CF2985A0A0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A morphometric study of mechanotransductively induced dermal neovascularization.
Journal
Plastic and Reconstructive Surgery
Author(s)
Erba P., Miele L.F., Adini A., Ackermann M., Lamarche J.M., Orgill B.D., D'Amato R.J., Konerding M.A., Mentzer S.J., Orgill D.P.
ISSN
1529-4242 (Electronic)
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
128
Number
4
Pages
288e-299e
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Abstract
BACKGROUND:: Mechanical stretch has been shown to induce vascular remodeling and increase vessel density, but the pathophysiologic mechanisms and the morphologic changes induced by tensile forces to dermal vessels are poorly understood.
METHODS:: A custom computer-controlled stretch device was designed and applied to the backs of C57BL/6 mice (n = 38). Dermal and vascular remodeling was studied over a 7-day period. Corrosion casting and three-dimensional scanning electron microscopy and CD31 staining were performed to analyze microvessel morphology. Hypoxia was assessed by immunohistochemistry. Western blot analysis of vascular endothelial growth factor (VEGF) and mRNA expression of VEGF receptors was performed.
RESULTS:: Skin stretching was associated with increased angiogenesis as demonstrated by CD31 staining and vessel corrosion casting where intervascular distance and vessel diameter were decreased (p < 0.01). Immediately after stretching, VEGF dimers were increased. Messenger RNA expression of VEGF receptor 1, VEGF receptor 2, neuropilin 1, and neuropilin 2 was increased starting as early as 2 hours after stretching. Highly proliferating epidermal cells induced epidermal hypoxia starting at day 3 (p < 0.01).
CONCLUSIONS:: Identification of significant hypoxic cells occurred after identification of neovessels, suggesting an alternative mechanism. Increased expression of angiogenic receptors and stabilization of VEGF dimers may be involved in a mechanotransductive, prehypoxic induction of neovascularization.
Keywords
Animals, Blotting, Western, Cell Proliferation, Disease Models, Animal, Epidermis/physiology, Epithelial Cells/physiology, Immunohistochemistry, Male, Mice, Mice, Inbred C57BL, Neovascularization, Physiologic/physiology, RNA, Messenger/metabolism, Random Allocation, Regeneration/physiology, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Skin/blood supply, Skin/pathology, Statistics, Nonparametric, Stress, Mechanical, Tensile Strength, Vascular Endothelial Growth Factor A/metabolism
Pubmed
Web of science
Create date
07/10/2011 10:41
Last modification date
20/08/2019 15:30
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