Proton-observed carbon-edited NMR spectroscopy in strongly coupled second-order spin systems.

Details

Serval ID
serval:BIB_72C4A2A0E101
Type
Article: article from journal or magazin.
Collection
Publications
Title
Proton-observed carbon-edited NMR spectroscopy in strongly coupled second-order spin systems.
Journal
Magnetic Resonance in Medicine
Author(s)
Henry P.G., Marjanska M., Walls J.D., Valette J., Gruetter R., Ugurbil K.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Publication state
Published
Issued date
2006
Volume
55
Number
2
Pages
250-257
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Proton-observed carbon-edited (POCE) NMR spectroscopy is commonly used to measure 13C labeling with higher sensitivity compared to direct 13C NMR spectroscopy, at the expense of spectral resolution. For weakly coupled first-order spin systems, the multiplet signal at a specific proton chemical shift in POCE spectra directly reflects 13C enrichment of the carbon attached to this proton. The present study demonstrates that this is not necessarily the case for strongly coupled second-order spin systems. In such cases NMR signals can be detected in the POCE spectra even at chemical shifts corresponding to protons bound to 12C. This effect is demonstrated theoretically with density matrix calculations and simulations, and experimentally with measured POCE spectra of [3-13C]glutamate.
Keywords
Carbon Isotopes, Computer Simulation, Glutamic Acid/chemistry, Magnetic Resonance Spectroscopy/methods, Protons, Sensitivity and Specificity
Pubmed
Web of science
Open Access
Yes
Create date
04/08/2010 16:28
Last modification date
08/05/2019 20:23
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