Localization of alpha-isoforms of Na(+)-K(+)-ATPase in rat kidney by in situ hybridization

Details

Serval ID
serval:BIB_72B177EEFC2F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Localization of alpha-isoforms of Na(+)-K(+)-ATPase in rat kidney by in situ hybridization
Journal
American Journal of Physiology
Author(s)
Farman  N., Corthesy-Theulaz  I., Bonvalet  J. P., Rossier  B. C.
ISSN
0363-6143
Publication state
Published
Issued date
03/1991
Volume
260
Number
3 Pt 1
Pages
C468-74
Notes
Journal Article --- Old month value: Mar
Abstract
The expression of the three alpha-isoforms of Na(+)-K(+)-adenosine triphosphatase (ATPase) was examined in rat brain and rat kidney by Northern blot analysis. All three isoforms were detected in brain tissue while alpha 1-isoform was observed in whole kidney, suggesting that either this isoform was solely expressed in this organ or that alpha 2- and/or alpha 3-isoforms were not detected only because of their restricted distribution among a minority of specialized tubular cells. To distinguish between these two possibilities, in situ hybridization with rat alpha 1-, alpha 2-, and alpha 3-ATPase cRNA was performed on rat kidney sections. Results show that alpha 1-isoform expression largely predominates in the loop of Henle, distal tubule, and cortical collecting tubule. The labeling was drastically reduced by preincubation of sections with RNase. A sense cRNA probe, used as control, did not hybridize. With alpha 2- and alpha 3-probes, the labeling was low and uniformly distributed. In contrast, these two isoforms were clearly expressed in the brain, together with alpha 1. We conclude that only alpha 1-isoform of the Na(+)-K(+)-ATPase is detectable along the rat nephron. Its expression predominates in the tubular segments known to have a high Na(+)-K(+)-ATPase activity.
Keywords
Animals Antisense Elements (Genetics) Blotting, Northern Brain/enzymology DNA/*analysis/genetics Female Isoenzymes/*genetics Kidney/cytology/*enzymology Kidney Cortex/enzymology Kidney Glomerulus/enzymology Kidney Medulla/enzymology Kidney Tubules/enzymology Nucleic Acid Hybridization RNA Probes Rats Rats, Inbred Strains Sodium-Potassium-Exchanging ATPase/*genetics
Pubmed
Web of science
Create date
24/01/2008 13:00
Last modification date
20/08/2019 14:30
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