Identification of three Sorex species with microsatellite markers
Details
Serval ID
serval:BIB_729C15C61AE1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of three Sorex species with microsatellite markers
Journal
Mammalia
ISSN
0025-1461
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
67
Number
2
Pages
245-252
Language
english
Abstract
Three sibling species of shrews, the common shrew (Sorex araneus), the Valais shrew (S. antinorii) and the Jersey shrew (S. coronatus) are morphologically similar. Different techniques based on karyorypes, morphology, biochemistry and genetic markers have been developed to identify individuals from these taxa. In this paper, we have used multiple microsatellite markers (L13, L14 and L99) to identify 55 dead animals coming from the Tarentaise Valley in France. As some individuals showed an unclear pattern with loci previously thought to be diagnostic (Lugon-Moulin et al. 2000), we have used morphologic measurements (Hausser et al. 1991) to confirm the status of these animals. This analysis clearly showed the limitations of the use of genetic diagnostic markers that have been designed in local populations and then applied to a wider scale. Even if these markers have great advantages over other techniques (i.e. simple to use and do not require samples from living animals), they should always be used with caution. There is always a risk of a locus not being diagnostic in the sampling region or in finding individuals with hybrid genotypes. Additional genetic markers should then be used, simultaneously with other identification techniques, to be sure of the status of the individuals.
Keywords
common shrew, Sorex species identification, diagnostic markers, microsatellites
Web of science
Create date
24/01/2008 20:02
Last modification date
20/08/2019 15:30