Engineered fluidic device to achieve multiplexed monitoring of cell cultures with digital holographic microscopy.

Details

Serval ID
serval:BIB_727953576678
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Engineered fluidic device to achieve multiplexed monitoring of cell cultures with digital holographic microscopy.
Journal
Optics express
Author(s)
Bélanger E., Benadiba C., Rioux-Pellerin É., Becq F., Jourdain P., Marquet P.
ISSN
1094-4087 (Electronic)
ISSN-L
1094-4087
Publication state
Published
Issued date
03/01/2022
Peer-reviewed
Oui
Volume
30
Number
1
Pages
414-426
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
We present a low-cost, 3D-printed, and biocompatible fluidic device, engineered to produce laminar and homogeneous flow over a large field-of-view. Such a fluidic device allows us to perform multiplexed temporal monitoring of cell cultures compatible with the use of various pharmacological protocols. Therefore, specific properties of each of the observed cell cultures can be discriminated simultaneously during the same experiment. This was illustrated by monitoring the agonists-mediated cellular responses, with digital holographic microscopy, of four different cell culture models of cystic fibrosis. Quantitatively speaking, this multiplexed approach provides a time saving factor of around four to reveal specific cellular features.
Keywords
Cell Culture Techniques/methods, Holography/methods, Microscopy/methods
Pubmed
Web of science
Open Access
Yes
Funding(s)
University of Lausanne
Create date
14/01/2022 17:41
Last modification date
18/11/2023 7:09
Usage data