HdaB: a novel and conserved DnaA-related protein that targets the RIDA process to stimulate replication initiation.

Details

Serval ID
serval:BIB_72742351BADF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
HdaB: a novel and conserved DnaA-related protein that targets the RIDA process to stimulate replication initiation.
Journal
Nucleic acids research
Author(s)
Frandi A., Collier J.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
18/03/2020
Peer-reviewed
Oui
Volume
48
Number
5
Pages
2412-2423
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Exquisite control of the DnaA initiator is critical to ensure that bacteria initiate chromosome replication in a cell cycle-coordinated manner. In many bacteria, the DnaA-related and replisome-associated Hda/HdaA protein interacts with DnaA to trigger the Regulatory Inactivation of DnaA (RIDA) and prevent over-initiation events. In the Caulobacter crescentus Alphaproteobacterium, the RIDA process also targets DnaA for its rapid proteolysis by Lon. The impact of the RIDA process on adaptation of bacteria to changing environments remains unexplored. Here, we identify a novel and conserved DnaA-related protein, named HdaB, and show that homologs from three different Alphaproteobacteria can inhibit the RIDA process, leading to over-initiation and cell death when expressed in actively growing C. crescentus cells. We further show that HdaB interacts with HdaA in vivo, most likely titrating HdaA away from DnaA. Strikingly, we find that HdaB accumulates mainly during stationary phase and that it shortens the lag phase upon exit from stationary phase. Altogether, these findings suggest that expression of hdaB during stationary phase prepares cells to restart the replication of their chromosome as soon as conditions improve, a situation often met by free-living or facultative intracellular Alphaproteobacteria.
Keywords
Bacterial Proteins/metabolism, Caulobacter crescentus/cytology, Caulobacter crescentus/growth & development, Caulobacter crescentus/metabolism, Cell Death, Cell Division, Chromosomes, Bacterial/metabolism, Conserved Sequence, DNA Replication, Models, Biological, Mutation/genetics, Protein Binding
Pubmed
Web of science
Open Access
Yes
Create date
03/01/2020 14:01
Last modification date
18/06/2020 6:21
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