Analysis of the oxidative stress regulation of the Candida albicans transcription factor, Cap1p

Details

Serval ID
serval:BIB_7204C19A94DA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Analysis of the oxidative stress regulation of the Candida albicans transcription factor, Cap1p
Journal
Molecular Microbiology
Author(s)
Zhang  X., De Micheli  M., Coleman  S. T., Sanglard  D., Moye-Rowley  W. S.
ISSN
0950-382X (Print)
Publication state
Published
Issued date
05/2000
Volume
36
Number
3
Pages
618-29
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: May
Abstract
CAP1 encodes a basic region-leucine zipper (bZip) transcriptional regulatory protein that is required for oxidative stress tolerance in Candida albicans. Cap1p is a homologue of a Saccharomyces cerevisiae bZip transcription factor designated Yap1p that is both required for oxidative stress tolerance and localized to the nucleus in response to the presence of oxidants. Oxidant-regulated localization of Yap1p to the nucleus requires the presence of a carboxy-terminal cysteine residue (C629) that is conserved in Cap1p as C477. To examine the role of this conserved cysteine residue, C477 was replaced with an alanine residue. This mutant protein, C477A Cap1p, was analysed for its behaviour both in S. cerevisiae and C. albicans. Wild type and C477A Cap1p were able to complement the oxidant hypersensitivity of a Deltayap1 S. cerevisiae strain. Whereas a Yap1p-responsive lacZ fusion gene was oxidant inducible in the presence of YAP1, the C. albicans Cap1p derivatives were not oxidant responsive in S. cerevisiae. Introduction of wild type and C477A Cap1p-expressing plasmids into C. albicans produced differential resistance to oxidants. Glutathione reductase activity was found to be inducible by oxidants in the presence of Cap1p but was constitutively elevated in the presence of C477A Cap1p. Western blot assays indicate Cap1p is post-translationally regulated by oxidants. Green fluorescent protein fusions to CAP1 showed that this protein is localized to the nucleus only in the presence of oxidants while C477A Cap1p is constitutively nuclear localized. Directly analogous to S. cerevisiae Yap1p, regulated nuclear localization of C. albicans Cap1p is crucial for its normal function.
Keywords
Basic-Leucine Zipper Transcription Factors Binding Sites Candida albicans/genetics/*physiology DNA-Binding Proteins/chemistry/metabolism Fungal Proteins/chemistry/metabolism *Gene Expression Regulation, Fungal Genetic Complementation Test Leucine Zippers *Oxidative Stress Phenotype Recombinant Proteins/metabolism Saccharomyces cerevisiae/physiology *Saccharomyces cerevisiae Proteins Transcription Factors/chemistry/*genetics/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:40
Last modification date
20/08/2019 14:30
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