Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia.

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Version: author
Serval ID
serval:BIB_71AEC25F7A25
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Endogenous angiotensinergic system in neurons of rat and human trigeminal ganglia.
Journal
Regulatory Peptides
Author(s)
Imboden H., Patil J., Nussberger J., Nicoud F., Hess B., Ahmed N., Schaffner T., Wellner M., Müller D., Inagami T., Senbonmatsu T., Pavel J., Saavedra J.M.
ISSN
0167-0115
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
154
Number
1-3
Pages
23-31
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
To clarify the role of Angiotensin II (Ang II) in the sensory system and especially in the trigeminal ganglia, we studied the expression of angiotensinogen (Ang-N)-, renin-, angiotensin converting enzyme (ACE)- and cathepsin D-mRNA, and the presence of Ang II and substance P in the rat and human trigeminal ganglia. The rat trigeminal ganglia expressed substantial amounts of Ang-N- and ACE mRNA as determined by quantitative real time PCR. Renin mRNA was untraceable in rat samples. Cathepsin D was detected in the rat trigeminal ganglia indicating the possibility of existence of pathways alternative to renin for Ang I formation. In situ hybridization in rat trigeminal ganglia revealed expression of Ang-N mRNA in the cytoplasm of numerous neurons. By using immunocytochemistry, a number of neurons and their processes in both the rat and human trigeminal ganglia were stained for Ang II. Post in situ hybridization immunocytochemistry reveals that in the rat trigeminal ganglia some, but not all Ang-N mRNA-positive neurons marked for Ang II. In some neurons Substance P was found colocalized with Ang II. Angiotensins from rat trigeminal ganglia were quantitated by radioimmunoassay with and without prior separation by high performance liquid chromatography. Immunoreactive angiotensin II (ir-Ang II) was consistently present and the sum of true Ang II (1-8) octapeptide and its specifically measured metabolites were found to account for it. Radioimmunological and immunocytochemical evidence of ir-Ang II in neuronal tissue is compatible with Ang II as a neurotransmitter. In conclusion, these results suggest that Ang II could be produced locally in the neurons of rat trigeminal ganglia. The localization and colocalization of neuronal Ang II with Substance P in the trigeminal ganglia neurons may be the basis for a participation and function of Ang II in the regulation of nociception and migraine pathology.
Keywords
Adult, Angiotensin I/analysis, Angiotensin II/analysis, Angiotensinogen/analysis, Angiotensinogen/genetics, Animals, Humans, Immunohistochemistry, In Situ Hybridization, Male, Neurons/metabolism, RNA, Messenger/metabolism, Radioimmunoassay, Rats, Rats, Inbred WKY, Trigeminal Ganglion/metabolism
Pubmed
Web of science
Create date
15/02/2010 13:54
Last modification date
20/08/2019 14:30
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