Tumor-infiltrating dendritic cell precursors recruited by a beta-defensin contribute to vasculogenesis under the influence of Vegf-A.

Details

Serval ID
serval:BIB_7186A8D7E33C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Tumor-infiltrating dendritic cell precursors recruited by a beta-defensin contribute to vasculogenesis under the influence of Vegf-A.
Journal
Nature Medicine
Author(s)
Conejo-Garcia J.R., Benencia F., Courreges M.C., Kang E., Mohamed-Hadley A., Buckanovich R.J., Holtz D.O., Jenkins A., Na H., Zhang L., Wagner D.S., Katsaros D., Caroll R., Coukos G.
ISSN
1078-8956 (Print)
ISSN-L
1078-8956
Publication state
Published
Issued date
2004
Volume
10
Number
9
Pages
950-958
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
The involvement of immune mechanisms in tumor angiogenesis is unclear. Here we describe a new mechanism of tumor vasculogenesis mediated by dendritic cell (DC) precursors through the cooperation of beta-defensins and vascular endothelial growth factor-A (Vegf-A). Expression of mouse beta-defensin-29 recruited DC precursors to tumors and enhanced tumor vascularization and growth in the presence of increased Vegf-A expression. A new leukocyte population expressing DC and endothelial markers was uncovered in mouse and human ovarian carcinomas coexpressing Vegf-A and beta-defensins. Tumor-infiltrating DCs migrated to tumor vessels and independently assembled neovasculature in vivo. Bone marrow-derived DCs underwent endothelial-like differentiation ex vivo, migrated to blood vessels and promoted the growth of tumors expressing high levels of Vegf-A. We show that beta-defensins and Vegf-A cooperate to promote tumor vasculogenesis by carrying out distinct tasks: beta-defensins chemoattract DC precursors through CCR6, whereas Vegf-A primarily induces their endothelial-like specialization and migration to vessels, which is mediated by Vegf receptor-2.
Keywords
Angiogenesis Inducing Agents/immunology, Animals, Cell Differentiation/immunology, Cell Movement/immunology, Collagen, Dendritic Cells/immunology, Dendritic Cells/metabolism, Drug Combinations, Flow Cytometry, Histological Techniques, Immunoblotting, Laminin, Mice, Neovascularization, Pathologic/immunology, Proteoglycans, Receptors, Chemokine/metabolism, Vascular Endothelial Growth Factor A/metabolism, beta-Defensins/immunology, beta-Defensins/metabolism
Pubmed
Web of science
Create date
14/10/2014 11:43
Last modification date
20/08/2019 14:30
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