Criblamydia sequanensis, a new intracellular Chlamydiales isolated from Seine river water using amoebal co-culture

Details

Serval ID
serval:BIB_711854D4BE19
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Criblamydia sequanensis, a new intracellular Chlamydiales isolated from Seine river water using amoebal co-culture
Journal
Environmental Microbiology
Author(s)
Thomas  V., Casson  N., Greub  G.
ISSN
1462-2912 (Print)
Publication state
Published
Issued date
12/2006
Volume
8
Number
12
Pages
2125-35
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
Accumulating evidence supports a role for Chlamydia-related organisms as emerging pathogens for human and animals. Assessment of their pathogenicity requires strain availability, at least for animal models and serological studies. As these obligate intracellular species are able to grow inside amoebae, we used co-culture with Acanthamoeba castellanii in an attempt to recover new Chlamydia-related species from river water. We isolated two strains from eight water samples. The first strain is a new Parachlamydia acanthamoebae strain that differs from previously described isolates by only two bases in the complete 16S rRNA gene sequence. The second isolate is the first representative of a new Chlamydiales family, as demonstrated by genetic and phylogenetic analyses of the 16S rRNA, 23S rRNA, ADP/ATP translocase and RnpB encoding genes. Using fluorescent in situ hybridization and electron microscopy, we demonstrated that it grows in high numbers in amoebae, where it exhibits a Chlamydia-like developmental cycle with reticulate bodies and star-like elementary bodies. Based on these results, we propose to name this new species 'Criblamydia sequanensis'. This work confirmed that amoebal co-culture is a relevant method to isolate new chlamydiae, and that it can be successfully applied to ecosystems colonized with a complex microbial community.
Keywords
Amoeba/*microbiology Animals Chlamydiaceae/genetics/*isolation & purification Coculture Techniques/methods France Fresh Water/*microbiology Microscopy, Confocal Microscopy, Electron, Transmission Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics
Pubmed
Web of science
Create date
25/01/2008 14:28
Last modification date
20/08/2019 14:29
Usage data