Detection of human immunodeficiency virus type 1 (HIV-1) RNA in pools of sera negative for antibodies to HIV-1 and HIV-2.

Details

Serval ID
serval:BIB_7117
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Detection of human immunodeficiency virus type 1 (HIV-1) RNA in pools of sera negative for antibodies to HIV-1 and HIV-2.
Journal
Journal of Clinical Microbiology
Author(s)
Morandi P.A., Schockmel G.A., Yerly S., Burgisser P., Erb P., Matter L., Sitavanc R., Perrin L.
ISSN
0095-1137 (Print)
ISSN-L
0095-1137
Publication state
Published
Issued date
1998
Volume
36
Number
6
Pages
1534-1538
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
A total of 234 pools were prepared from 10,692 consecutive serum samples negative for antibodies to human immunodeficiency virus type 1 (HIV-1) and HIV-2 collected at five virological laboratories (average pool size, 45 serum samples). Pools were screened for the presence of HIV-1 RNA by a modified commercial assay (Amplicor HIV-1 Monitor test) which included an additional polyethylene glycol (PEG) precipitation step prior to purification of viral RNA (PEG Amplicor assay). The sensitivity of this assay for HIV-1 RNA detection in individual serum samples within pools matches that of standard commercial assays for individual serum samples, i.e., 500 HIV-1 RNA copies per ml. Five pools were identified as positive, and each one contained one antibody-negative, HIV-1 RNA-positive serum sample, corresponding to an average of 1 infected sample per 2,138 serum samples. Retrospective analysis revealed that the five HIV-1 RNA-positive specimens originated from individuals who had symptomatic primary HIV-1 infection at the time of sample collection and who were also positive for p24 antigenemia. We next assessed the possibility of performing the prepurification step by high-speed centrifugation (50,000 x g for 80 min) of 1.5-ml pools containing 25 microl of 60 individual serum samples, of which only 1 contained HIV-1 RNA (centrifugation Amplicor assay). The sensitivity of this assay also matches the sensitivities of standard commercial assays for HIV-1 RNA detection in individual serum samples. The results demonstrate that both assays with pooled sera can be applied to the screening of large numbers of serum samples in a time- and cost-efficient manner.
Keywords
Centrifugation, Chemical Precipitation, Evaluation Studies as Topic, HIV Antibodies/blood, HIV Infections/blood, HIV Infections/diagnosis, HIV Seronegativity, HIV-1/genetics, HIV-1/immunology, HIV-2/immunology, Humans, Polyethylene Glycols, Polymerase Chain Reaction/methods, RNA, Viral/blood, Reagent Kits, Diagnostic, Sensitivity and Specificity
Pubmed
Web of science
Create date
19/11/2007 12:44
Last modification date
20/08/2019 14:29
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