Detection of antibodies to central nervous system antigens by solid phase radioimmunoassay.

Details

Serval ID
serval:BIB_70F2BEE6E693
Type
Article: article from journal or magazin.
Collection
Publications
Title
Detection of antibodies to central nervous system antigens by solid phase radioimmunoassay.
Journal
Journal of Neuroscience Research
Author(s)
Linthicum D.S., Mackay I.R., Wilson A., Horvath L.B., Carnegie P.R.
ISSN
0360-4012 (Print)
ISSN-L
0360-4012
Publication state
Published
Issued date
1981
Volume
6
Number
5
Pages
567-578
Language
english
Abstract
A solid phase radioimmunoassay is described which employs 125I-protein-A to detect the presence of antibodies against a panel of cellular and soluble central nervous system (CNS) specific antigens coated onto polyvinylchloride Microtiter plates. Serum antibodies from rabbits immunized against myelin, myelin basic protein (MBP), glial acidic fibrillary protein (GFAP), astroglioma cells, and cerebellar cells were easily detected, and high specificity for each antiserum and antigen was also demonstrable. The assay is applicable to cerebrospinal fluid (CSF) from patients with neurological diseases to detect antibodies against CNS-specific antigens. The assay should be useful for examining cell lines derived from CNS tissue for the presence of brain proteins.
Keywords
Adolescent, Adult, Aged, Animals, Antigens/immunology, Astrocytes/immunology, Autoantibodies/cerebrospinal fluid, Cattle, Central Nervous System/immunology, Cerebellum/immunology, Child, Preschool, Female, Glial Fibrillary Acidic Protein, Humans, Infant, Male, Mice, Middle Aged, Myelin Basic Protein/immunology, Myelin Sheath/immunology, Nerve Tissue Proteins/immunology, Nervous System Diseases/immunology, Rabbits/immunology, Radioimmunoassay, Rats
Pubmed
Web of science
Create date
10/04/2013 10:16
Last modification date
20/08/2019 14:29
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