Velocity estimates for signal propagation leading to systemic jasmonic acid accumulation in wounded Arabidopsis.

Details

Serval ID
serval:BIB_70C3967F340E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Velocity estimates for signal propagation leading to systemic jasmonic acid accumulation in wounded Arabidopsis.
Journal
Journal of Biological Chemistry
Author(s)
Glauser G., Dubugnon L., Mousavi S.A., Rudaz S., Wolfender J.L., Farmer E.E.
ISSN
1083-351X[electronic], 0021-9258[linking]
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
284
Number
50
Pages
34506-34513
Language
english
Abstract
The wound response prohormone jasmonic acid (JA) accumulates rapidly in tissues both proximal and distal to injury sites in plants. Using quantitative liquid chromatography-mass spectrometry after flash freezing of tissues, we found that JA accumulated within 30 s of injury in wounded Arabidopsis leaves (p = 3.5 e(-7)). JA augmentation distal to wounds was strongest in unwounded leaves with direct vascular connections to wounded leaves wherein JA levels increased significantly within 120 s of wounding (p = 0.00027). This gave conservative and statistically robust temporal boundaries for the average velocity of the long distance signal leading to distal JA accumulation in unwounded leaves of 3.4-4.5 cm min(-1). Like JA, transcripts of the JA synthesis gene LIPOXYGENASE2 (LOX2) and the jasmonate response gene JAZ10.3 also accumulated to higher levels in directly interconnected leaves than in indirectly connected leaves. JA accumulation in a lox2-1 mutant plant was initiated rapidly after wounding then slowed progressively compared with the wild type (WT). Despite this, JAZ10.3 expression in the two genotypes was similar. Free cyclopentenone jasmonate levels were similar in both resting WT and lox2-1. In contrast, bound cyclopentenone jasmonates (arabidopsides) were far lower in lox2-1 than in the WT. The major roles of LOX2 are to generate arabidopsides and the large levels of JA that accumulate proximal to the wound. LOX2 is not essential for some of the most rapid events elicited by wounding.
Keywords
Animals, Arabidopsis/anatomy & histology, Arabidopsis/genetics, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Cyclopentanes/metabolism, Freezing, Gene Expression Regulation, Plant, Lipoxygenase/genetics, Lipoxygenase/metabolism, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Oxylipins/metabolism, Plant Growth Regulators/metabolism, Plant Leaves/metabolism, Plant Leaves/parasitology, Signal Transduction/physiology, Spodoptera/metabolism
Pubmed
Web of science
Create date
13/01/2010 13:55
Last modification date
20/08/2019 14:29
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