Small intestinal resident eosinophils maintain gut homeostasis following microbial colonization.

Details

Serval ID
serval:BIB_70C1B74BEBAC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Small intestinal resident eosinophils maintain gut homeostasis following microbial colonization.
Journal
Immunity
Author(s)
Ignacio A., Shah K., Bernier-Latmani J., Köller Y., Coakley G., Moyat M., Hamelin R., Armand F., Wong N.C., Ramay H., Thomson C.A., Burkhard R., Wang H., Dufour A., Geuking M.B., McDonald B., Petrova T.V., Harris N.L., McCoy K.D.
ISSN
1097-4180 (Electronic)
ISSN-L
1074-7613
Publication state
Published
Issued date
12/07/2022
Peer-reviewed
Oui
Volume
55
Number
7
Pages
1250-1267.e12
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The intestine harbors a large population of resident eosinophils, yet the function of intestinal eosinophils has not been explored. Flow cytometry and whole-mount imaging identified eosinophils residing in the lamina propria along the length of the intestine prior to postnatal microbial colonization. Microscopy, transcriptomic analysis, and mass spectrometry of intestinal tissue revealed villus blunting, altered extracellular matrix, decreased epithelial cell turnover, increased gastrointestinal motility, and decreased lipid absorption in eosinophil-deficient mice. Mechanistically, intestinal epithelial cells released IL-33 in a microbiota-dependent manner, which led to eosinophil activation. The colonization of germ-free mice demonstrated that eosinophil activation in response to microbes regulated villous size alterations, macrophage maturation, epithelial barrier integrity, and intestinal transit. Collectively, our findings demonstrate a critical role for eosinophils in facilitating the mutualistic interactions between the host and microbiota and provide a rationale for the functional significance of their early life recruitment in the small intestine.
Keywords
Animals, Communicable Diseases, Eosinophils, Homeostasis, Intestinal Mucosa, Intestine, Small, Mice, Microbiota, eosinophil, extracellular matrix, germ-free, intestinal barrier, microbiome, small intestine, tissue homeostasis, villous atrophy
Pubmed
Create date
05/07/2022 11:21
Last modification date
18/07/2022 5:35
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