Lack of H-2 restriction of the Plasmodium falciparum (NANP) sequence as multiple antigen peptide

Details

Serval ID
serval:BIB_70A5555302F3
Type
Article: article from journal or magazin.
Collection
Publications
Title
Lack of H-2 restriction of the Plasmodium falciparum (NANP) sequence as multiple antigen peptide
Journal
European Journal of Immunology
Author(s)
Pessi  A., Valmori  D., Migliorini  P., Tougne  C., Bianchi  E., Lambert  P. H., Corradin  G., Del Giudice  G.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
09/1991
Volume
21
Number
9
Pages
2273-6
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Abstract
The major surface antigen of malaria sporozoites, the circumsporozoite protein, contains a region of tandem amino acid repeats, which in the case of the human malaria parasite Plasmodium falciparum, consist of four amino acids Asn-Ala-Asn-Pro (NANP) repeated up to about 40 times. This repetitive sequence has been considered as the basis for the development of subunit vaccines against P. falciparum malaria. We and others had previously shown that synthetic and recombinant NANP peptides were immunogenic only in H-2b mice. In the present report we show that, when mice with different H-2 haplotypes are immunized with the repetitive NANP sequence incorporated in a synthetic branching multiple antigen peptide (MAP), all except one of the mouse strains tested mounted an anti-peptide antibody response. Such a response does not appear to be due to the peculiar assembly of the NANP sequence. In fact, MAP containing repetitive sequences from circumsporozoite proteins of other malaria parasites did not overcome the genetic restriction of the immune response to the linear peptides. These data show that in the case of the P. falciparum NANP repeats, their immunogenicity can be dramatically changed and increased when these peptides are assembled as MAP. This unexpected finding may be of interest in the design of synthetic candidate malaria vaccines.
Keywords
Amino Acid Sequence Animals Antigens, Protozoan/*immunology Enzyme-Linked Immunosorbent Assay H-2 Antigens/*immunology Immunization Immunoglobulin G/biosynthesis Malaria/genetics/immunology Mice Mice, Inbred Strains Molecular Sequence Data Plasmodium berghei/immunology Plasmodium falciparum/*immunology Plasmodium malariae/immunology Plasmodium vivax/immunology *Protozoan Proteins Repetitive Sequences, Nucleic Acid/*immunology
Pubmed
Web of science
Create date
24/01/2008 15:55
Last modification date
20/08/2019 15:29
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