Use of ion trap gas chromatography-multiple mass spectrometry for the detection and confirmation of 3 ' hydroxystanozolol at trace levels in urine for doping control

Details

Serval ID
serval:BIB_7036E66D7DA4
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Institution
Title
Use of ion trap gas chromatography-multiple mass spectrometry for the detection and confirmation of 3 ' hydroxystanozolol at trace levels in urine for doping control
Journal
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Author(s)
Mateus-Avois  L., Mangin  P., Saugy  M.
ISSN
1570-0232
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
816
Number
1-2
Pages
193-201
Abstract
Stanozolol, a synthetic anabolic androgenic steroid, is often abused in sports to enhance performance. Consequently, the anti-doping laboratories daily screen for its metabolites (3'hydroxystanozolol and 4beta hydroxystanozolol) in all urines, mainly by GC-MS, after enzymatic hydrolysis and TMS derivatization. A sensitive and specific method by GC-MS3 has been developed for the identification in urine of 3'hydroxystanozolol at trace levels. Full mass spectra and diagnostic ions are presented and a case report is commented. In this case, it was possible to attest the presence of a low concentration of stanozolol metabolite in a sample obtained from a competition test. This would have not been possible with other analytical techniques used in the laboratory. Through this case report, it was also possible to demonstrate the importance of sampling and the difficulties that has to face the laboratory when the pre-analytical step is not correctly performed.
Pubmed
Web of science
Create date
08/02/2008 17:44
Last modification date
20/08/2019 14:28
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