Mechanical characterization of collagen fibers and scaffolds for tissue engineering.

Details

Serval ID
serval:BIB_6FFEDAB15980
Type
Article: article from journal or magazin.
Collection
Publications
Title
Mechanical characterization of collagen fibers and scaffolds for tissue engineering.
Journal
Biomaterials
Author(s)
Gentleman E., Lay A.N., Dickerson D.A., Nauman E.A., Livesay G.A., Dee K.C.
ISSN
0142-9612 (Print)
ISSN-L
0142-9612
Publication state
Published
Issued date
09/2003
Peer-reviewed
Oui
Volume
24
Number
21
Pages
3805-3813
Language
english
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Abstract
Engineered tissues must utilize scaffolding biomaterials that support desired cellular functions and possess or can develop appropriate mechanical characteristics. This study assessed properties of collagen as a scaffolding biomaterial for ligament replacements. Mechanical properties of extruded bovine achilles tendon collagen fibers were significantly affected by fiber diameter, with smaller fibers displaying higher tangent moduli and peak stresses. Mechanical properties of 125 micrometer-diameter extruded fibers (tangent modulus of 359.6+/-28.4MPa; peak stress of 36.0+/-5.4MPa) were similar to properties reported for human ligaments. Scaffolds of extruded fibers did not exhibit viscoelastic creep properties similar to natural ligaments. Collagen fibers from rat tail tendon (a well-studied comparison material) displayed characteristic strain-softening behavior, and scaffolds of rat tail fibers demonstrated a non-intuitive relationship between tangent modulus and specimen length. Composite scaffolds (extruded collagen fibers cast within a gel of Type I rat tail tendon collagen) were maintained with and without fibroblasts under standard culture conditions for 25 days; cell-incorporated scaffolds displayed significantly higher tangent moduli and peak stresses than those without cells. Because tissue-engineered products must possess appropriate mechanical as well as biological/chemical properties, data from this study should help enable the development of improved tissue analogues.
Keywords
Animals, Biocompatible Materials/chemistry, Cattle, Collagen/chemistry, Fibroblasts/metabolism, Humans, Ligaments/chemistry, Ligaments/metabolism, Materials Testing, Mechanics, Rats, Tensile Strength, Time Factors, Tissue Engineering/methods
Pubmed
Web of science
Create date
12/01/2024 11:14
Last modification date
13/01/2024 8:10
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