Alkylamine derivatives of cytochrome c. Comparison with other lysine-modified analogues illuminates structure/function relations in the protein

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Serval ID
serval:BIB_6FEC42E0BEAA
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Alkylamine derivatives of cytochrome c. Comparison with other lysine-modified analogues illuminates structure/function relations in the protein
Journal
European Journal of Biochemistry
Author(s)
Wallace  C. J., Corthesy  B. E.
ISSN
0014-2956 (Print)
Publication state
Published
Issued date
12/1987
Volume
170
Number
1-2
Pages
293-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 30
Abstract
For investigations of the functional roles of the lysine residues of cytochrome c, analogues in which these residues are modified without charge loss are highly desirable. The 19 lysine residues of the horse heart protein have been modified by reductive alkylation. Two analogues were prepared, using formaldehyde and acetone as the dialkylating and monoalkylating reagent respectively. Modification was shown to be clean and quantitative. Characterisation of the alkylamine derivatives by physicochemical measurements and biological activity determinations was carried out. The potential of these analogues in structure/function studies of cytochrome c is discussed. It is illustrated by their use, in conjunction with other lysine-modified derivatives, to investigate the extent to which surface charge determines redox potential, and to study the physicochemical changes that accompany rising pH. In the latter case the observed phenomena are not as closely correlated as previously thought, suggesting that there is a complex set of rearrangements of structure underlying the functional changes. The data confirm that modification of the lysine residues influences these changes. These residues participate in numerous surface intramolecular links, so the lack of correlation may be explained if each of the changing parameters were under the influence of a different set of residues. However, neither a lysine residue, nor a histidine residue directly displaces methionine from the sixth coordination position of the haem iron at alkaline pH.
Keywords
Alkylation Amines Amino Acids/analysis Animals Cytochrome c Group/isolation & purification/*metabolism Horses Kinetics Lysine Oxidation-Reduction Structure-Activity Relationship Tuna
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:53
Last modification date
20/08/2019 14:28
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