Microtubule affinity chromatography: a new technique for isolating microtubule-binding proteins from rat pancreas.

Details

Serval ID
serval:BIB_6FC73CA57DA7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Microtubule affinity chromatography: a new technique for isolating microtubule-binding proteins from rat pancreas.
Journal
Cellular and Molecular Biology
Author(s)
Michalik L., Vanier M.T., Launay J.F.
ISSN
0145-5680 (Print)
ISSN-L
0145-5680
Publication state
Published
Issued date
1991
Volume
37
Number
8
Pages
805-811
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
We have developed an affinity chromatography method for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts of rat pancreas. Among the ten proteins which copurify with pancreas tubulin on a colchicine derivatives-affinity chromatography, three polypeptides of respectively 58, 55 and 48 kDa strongly bind to the microtubule affinity column. To begin to characterize these proteins, we have generated polyclonal antibodies against tau polypeptides from brains of immature chicken or rat. As judged by immunoblots, the three polypeptides seem to be immunologically related to the tau proteins previously localized in brain.
Keywords
Animals, Chromatography, Affinity/methods, Colchicine/metabolism, Microtubule-Associated Proteins/isolation & purification, Microtubules/metabolism, Pancreas/chemistry, Rats, tau Proteins
Pubmed
Web of science
Create date
24/01/2008 16:44
Last modification date
20/08/2019 15:28
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