Demonstration of cross-reactive antibodies to smooth gram-negative bacteria in antiserum to Escherichia coli J5

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Serval ID
serval:BIB_6F758B9936FB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Demonstration of cross-reactive antibodies to smooth gram-negative bacteria in antiserum to Escherichia coli J5
Journal
Journal of Infectious Diseases
Author(s)
Baumgartner  J. D., O'Brien  T. X., Kirkland  T. N., Glauser  M. P., Ziegler  E. J.
ISSN
0022-1899
Publication state
Published
Issued date
07/1987
Peer-reviewed
Oui
Volume
156
Number
1
Pages
136-43
Language
english
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jul
Abstract
We investigated the discrepancy between the broad cross-protection against gram-negative infections afforded by antiserum to Escherichia coli J5 and its apparently narrow cross-reactivity in vitro. Rabbits immunized with J5 bacteria produced antibodies to both the J5 lipopolysaccharide (LPS; titer by ELISA, 1:60,000) and LPS from the Re mutant of Salmonella minnesota (i.e., to the ketodeoxyoctonate [KDO] and lipid A determinants; titer, 1:3,200). In highly diluted antiserum, titers of antibody to J5 LPS were reduced by 28%-41% after adsorption with seven strains of smooth gram-negative bacteria and by only 4% after adsorption with the Re mutant. Smooth gram-negative bacteria adsorbed virtually all antibody to Re LPS. Therefore, rabbit antiserum to J5 contains type-specific antibodies to core determinants distal to KDO that can obscure highly cross-reactive antibodies to lipid A-KDO in vitro. Cross-reactive antibodies are demonstrable by adsorption with whole bacteria at limiting concentrations of antibody.
Keywords
Adsorption Animals Antibodies, Bacterial/analysis/*immunology Cross Reactions Enzyme-Linked Immunosorbent Assay Epitopes/analysis Escherichia coli/*immunology Gram-Negative Bacteria/*immunology Immune Sera/*immunology Lipopolysaccharides/immunology Rabbits
Pubmed
Web of science
Open Access
Yes
Create date
19/01/2008 18:12
Last modification date
01/10/2019 6:18
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