Appearance of granule-associated molecules during activation of cytolytic T-lymphocyte precursors by defined stimuli

Details

Serval ID
serval:BIB_6F45835755E1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Appearance of granule-associated molecules during activation of cytolytic T-lymphocyte precursors by defined stimuli
Journal
Immunology
Author(s)
Garcia-Sanz  J. A., Velotti  F., MacDonald  H. R., Masson  D., Tschopp  J., Nabholz  M.
ISSN
0019-2805 (Print)
Publication state
Published
Issued date
05/1988
Volume
64
Number
1
Pages
129-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Lysis of target cells by cytolytic T lymphocytes (CTL) is associated with the exocytosis of cytoplasmic granules. Purified granules from CTL cell lines contain a pore-forming protein (perforin), tree serine esterases, granzyme A (60,000 MW), granzyme B (29,000 MW), and granzyme C (27,000 MW). We have compared the kinetics of appearance of cytolytic activity with that of perforin and granzyme A activity during activation of lymphocytes from normal animals with leukoagglutinin (LA) and recombinant interleukin-2 (rIL-2). Unstimulated lymph node cells do not express any of these activities, which appear between Day 3 and Day 4 of stimulation and increase rapidly to reach a pronounced peak on Day 6. On Day 7 all the activities are considerably lower, even though the cells still proliferate exponentially. There is a good correlation between the kinetics of appearance of all of these activities. Using antisera against perforin and against granzyme C, one can detect positive cytoplasmic granules in a small fraction of cells on Day 3; by Day 5, 80-90% of the cells are stained. This proportion decreases again on Day 7.
Keywords
Animals Cells, Cultured Cytoplasmic Granules/metabolism Exocytosis Female Granzymes Hemolysis *Lymphocyte Activation *Membrane Glycoproteins Membrane Proteins/biosynthesis Mice Mice, Inbred Strains Pore Forming Cytotoxic Proteins Serine Endopeptidases/biosynthesis T-Lymphocytes, Cytotoxic/*immunology/metabolism Time Factors
Pubmed
Web of science
Create date
24/01/2008 15:18
Last modification date
20/08/2019 14:28
Usage data