Appearance of granule-associated molecules during activation of cytolytic T-lymphocyte precursors by defined stimuli
Details
Serval ID
serval:BIB_6F45835755E1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Appearance of granule-associated molecules during activation of cytolytic T-lymphocyte precursors by defined stimuli
Journal
Immunology
ISSN
0019-2805 (Print)
Publication state
Published
Issued date
05/1988
Volume
64
Number
1
Pages
129-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Lysis of target cells by cytolytic T lymphocytes (CTL) is associated with the exocytosis of cytoplasmic granules. Purified granules from CTL cell lines contain a pore-forming protein (perforin), tree serine esterases, granzyme A (60,000 MW), granzyme B (29,000 MW), and granzyme C (27,000 MW). We have compared the kinetics of appearance of cytolytic activity with that of perforin and granzyme A activity during activation of lymphocytes from normal animals with leukoagglutinin (LA) and recombinant interleukin-2 (rIL-2). Unstimulated lymph node cells do not express any of these activities, which appear between Day 3 and Day 4 of stimulation and increase rapidly to reach a pronounced peak on Day 6. On Day 7 all the activities are considerably lower, even though the cells still proliferate exponentially. There is a good correlation between the kinetics of appearance of all of these activities. Using antisera against perforin and against granzyme C, one can detect positive cytoplasmic granules in a small fraction of cells on Day 3; by Day 5, 80-90% of the cells are stained. This proportion decreases again on Day 7.
Keywords
Animals
Cells, Cultured
Cytoplasmic Granules/metabolism
Exocytosis
Female
Granzymes
Hemolysis
*Lymphocyte Activation
*Membrane Glycoproteins
Membrane Proteins/biosynthesis
Mice
Mice, Inbred Strains
Pore Forming Cytotoxic Proteins
Serine Endopeptidases/biosynthesis
T-Lymphocytes, Cytotoxic/*immunology/metabolism
Time Factors
Pubmed
Web of science
Create date
24/01/2008 16:18
Last modification date
20/08/2019 15:28