A radioimmunoassay for human insulin receptor correlation between insulin binding and receptor mass
Details
Serval ID
serval:BIB_6F392167591C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A radioimmunoassay for human insulin receptor correlation between insulin binding and receptor mass
Journal
Hormone and Metabolic Research
ISSN
0018-5043 (Print)
Publication state
Published
Issued date
03/1991
Volume
23
Number
3
Pages
117-21
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Abstract
We have developed a radioimmunoassay for human insulin receptor. Serum from a patient with Type B severe insulin resistance was used as anti-insulin receptor antiserum. Pure human placental insulin receptor was used as reference preparation and 125I labeled pure insulin receptor as trace. The radioimmunoassay was sensitive (limit of detection less than 17 fmol), reproducible (inter and intra-assay coefficients of variation 12.5% and 1.6% respectively) and specific (no crossreactivity with pure placental IGF-1 receptor, insulin and glucagon). The anti-insulin receptor antibody was, however, able to differentiate between insulin receptor from human placenta and from rat liver. To determine the number of insulin binding sites per receptor, we measured insulin binding (by insulin binding assay) and insulin receptor mass (by radioimmunoassay) in solubilized aliquots from 5 human placentas. The molar ratio of insulin binding to receptor mass was 0.86 +/- 0.12 when binding was determined with monoiodinated 125I-Tyr A 14-insulin. It was 1.94 +/- 0.27 when randomly iodinated 125I-insulin was used. In conclusion, using a sensitive, reproducible and specific radioimmunoassay, we have measured insulin receptor mass independent of insulin binding. Our data are most compatible with binding of one insulin molecule per human placental insulin receptor.
Keywords
Autoantibodies
Cell Membrane/chemistry/metabolism
Chromatography, Affinity
Female
Humans
Insulin/*metabolism
Insulin Resistance/immunology
Insulin-Like Growth Factor I/metabolism
Iodine Radioisotopes/diagnostic use
Kinetics
Molecular Weight
Placenta/chemistry/metabolism
Pregnancy
Radioimmunoassay/methods
Receptor, Insulin/*analysis/isolation & purification/metabolism
Receptors, Cell Surface/isolation & purification/metabolism
Receptors, Somatomedin
Pubmed
Web of science
Create date
24/01/2008 14:36
Last modification date
20/08/2019 15:28