UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics).

Details

Serval ID
serval:BIB_6F3829949833
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
UHPLC-HRMS Analysis for Steroid Profiling in Serum (Steroidomics).
Title of the book
Methods in molecular biology
Author(s)
Ponzetto F., Boccard J., Nicoli R., Kuuranne T., Saugy M., Rudaz S.
Publisher
Springer
ISBN
978-1-4939-7642-3
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
1738
Pages
261-278
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The extraction and untargeted UHPLC-HRMS analysis of endogenous steroids in serum samples is described in this protocol. The employed full-scan acquisition mode provides the adequate sensitivity to highlight the main endogenous steroids present in blood, including mineralocorticoids, progestogens , and androgens. Technical aspects for both chromatography and mass spectrometry are discussed in detail, together with a proposition of setup for sample sequence and data analysis. Furthermore, general comments are given to help the assessment of data quality and system performance.

Keywords
Extended steroid profiles, Steroidomics, Steroids, UHPLC-HRMS
Pubmed
Create date
17/04/2018 10:13
Last modification date
20/08/2019 14:28
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