Follicular Immune Landscaping Reveals a Distinct Profile of FOXP3hiCD4hi T Cells in Treated Compared to Untreated HIV.
Details
Serval ID
serval:BIB_6F34C11CEE24
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Follicular Immune Landscaping Reveals a Distinct Profile of FOXP3hiCD4hi T Cells in Treated Compared to Untreated HIV.
Journal
Vaccines
ISSN
2076-393X (Print)
ISSN-L
2076-393X
Publication state
Published
Issued date
12/08/2024
Peer-reviewed
Oui
Volume
12
Number
8
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
Follicular helper CD4 <sup>hi</sup> T cells (T <sub>FH</sub> ) are a major cellular pool for the maintenance of the HIV reservoir. Therefore, the delineation of the follicular (F)/germinal center (GC) immune landscape will significantly advance our understanding of HIV pathogenesis. We have applied multiplex confocal imaging, in combination with the relevant computational tools, to investigate F/GC in situ immune dynamics in viremic (vir-HIV), antiretroviral-treated (cART HIV) People Living With HIV (PLWH) and compare them to reactive, non-infected controls. Lymph nodes (LNs) from viremic and cART PLWH could be further grouped based on their T <sub>FH</sub> cell densities in high-T <sub>FH</sub> and low-T <sub>FH</sub> subgroups. These subgroups were also characterized by different in situ distributions of PD1 <sup>hi</sup> T <sub>FH</sub> cells. Furthermore, a significant accumulation of follicular FOXP3 <sup>hi</sup> CD4 <sup>hi</sup> T cells, which were characterized by a low scattering in situ distribution profile and strongly correlated with the cell density of CD8 <sup>hi</sup> T cells, was found in the cART-HIV low-TFH group. An inverse correlation between plasma viral load and LN GrzB <sup>hi</sup> CD8 <sup>hi</sup> T and CD16 <sup>hi</sup> CD15 <sup>lo</sup> cells was found. Our data reveal the complex GC immune landscaping in HIV infection and suggest that follicular FOXP3 <sup>hi</sup> CD4 <sup>hi</sup> T cells could be negative regulators of T <sub>FH</sub> cell prevalence in cART-HIV.
Keywords
CD4 T cells, HIV, germinal center, imaging
Pubmed
Open Access
Yes
Create date
03/09/2024 16:15
Last modification date
05/09/2024 10:07