Reproducibility of MDM2 and CDK4 staining in soft tissue tumors.

Details

Serval ID
serval:BIB_6EE4F3DCBC5C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Reproducibility of MDM2 and CDK4 staining in soft tissue tumors.
Journal
American Journal of Clinical Pathology
Author(s)
Binh M.B., Garau X.S., Guillou L., Aurias A., Coindre J.M.
ISSN
0002-9173
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
125
Number
5
Pages
693-697
Language
english
Abstract
MDM2 and CDK4 immunostaining can be useful adjuncts in diagnosing liposarcoma among soft tissue neoplasms. We examined the reproducibility of MDM2 and CDK4 staining between 2 laboratories and between tissue microarrays and whole tissue sections. Sixty-two soft tissue tumors were immunostained at the Bergonié Institute, Bordeaux, France, and the Curie Institute, Paris, France. We also examined 203 soft tissue neoplasms on standard tissue sections and tissue microarrays. There was high concordance of results obtained from the 2 laboratories (with 2 different pathologists) for MDM2 (kappa, 0.93) and CDK4 (kappa, 0.8) staining. There also was excellent concordance between results on tissue microarray and on whole tissue sections for MDM2 (kappa, 0.80) and CDK4 (kappa, 0.93). Immunostaining for MDM2 and CDK4 is a reproducible technique that may be exported to different laboratories for routine use. Tissue microarray is indicated for studying large series.
Keywords
Humans, Immunoenzyme Techniques, Keratins, Observer Variation, Proto-Oncogene Proteins c-mdm2, Reproducibility of Results, Retrospective Studies, Sarcoma, Soft Tissue Neoplasms, Tissue Array Analysis, Tumor Markers, Biological
Pubmed
Web of science
Open Access
Yes
Create date
29/01/2008 18:34
Last modification date
20/08/2019 14:28
Usage data