CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions.

Details

Serval ID
serval:BIB_6E8BCA9FDAE9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions.
Journal
Nature communications
Author(s)
Kaushal A., Mohana G., Dorier J., Özdemir I., Omer A., Cousin P., Semenova A., Taschner M., Dergai O., Marzetta F., Iseli C., Eliaz Y., Weisz D., Shamim M.S., Guex N., Lieberman Aiden E., Gambetta M.C.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
12/02/2021
Peer-reviewed
Oui
Volume
12
Number
1
Pages
1011
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Vertebrate genomes are partitioned into contact domains defined by enhanced internal contact frequency and formed by two principal mechanisms: compartmentalization of transcriptionally active and inactive domains, and stalling of chromosomal loop-extruding cohesin by CTCF bound at domain boundaries. While Drosophila has widespread contact domains and CTCF, it is currently unclear whether CTCF-dependent domains exist in flies. We genetically ablate CTCF in Drosophila and examine impacts on genome folding and transcriptional regulation in the central nervous system. We find that CTCF is required to form a small fraction of all domain boundaries, while critically controlling expression patterns of certain genes and supporting nervous system function. We also find that CTCF recruits the pervasive boundary-associated factor Cp190 to CTCF-occupied boundaries and co-regulates a subset of genes near boundaries together with Cp190. These results highlight a profound difference in CTCF-requirement for genome folding in flies and vertebrates, in which a large fraction of boundaries are CTCF-dependent and suggest that CTCF has played mutable roles in genome architecture and direct gene expression control during metazoan evolution.
Keywords
Animals, CCCTC-Binding Factor/genetics, CCCTC-Binding Factor/metabolism, Chromatin, Chromosomes/metabolism, Developmental Biology, Drosophila/genetics, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Female, Gene Knockout Techniques, Genome, Male, Microtubule-Associated Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
16/02/2021 9:45
Last modification date
27/03/2021 7:32
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