IL-4-mediated fine tuning of IL-12p70 production by human DC.

Details

Serval ID
serval:BIB_6E6909AF9F13
Type
Article: article from journal or magazin.
Collection
Publications
Title
IL-4-mediated fine tuning of IL-12p70 production by human DC.
Journal
European journal of immunology
Author(s)
Guenova E., Volz T., Sauer K., Kaesler S., Müller M.R., Wölbing F., Chen K., Schwärzler C., Brossart P., Röcken M., Biedermann T.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Publication state
Published
Issued date
11/2008
Peer-reviewed
Oui
Volume
38
Number
11
Pages
3138-3149
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
IL-4 is expressed at high levels in allergic diseases and dominates the early phases of multiple acquired immune responses. However, the precise role of IL-4 during early inflammation and its impact on the differentiation of newly recruited DC precursors remains elusive. In order to characterize the impact of IL-4 on the differentiation of human DC, we investigated the role of IL-4 on the differentiation of monocytes into DC. Human DC were differentiated from peripheral blood precursors under either low or high concentrations of IL-4. We analyzed their cytokine profile and capacity to polarize T-cell differentiation. Concentrations of 5 (low) and 50 (high) ng/mL IL-4 induced two distinct types of DC. DC differentiated under low-dose IL-4 (5 ng/mL) produced almost no IL-12p70, and primed naïve CD4+ T cells allowing IL-4 secretion and Th2 induction. In contrast, DC generated under high concentrations of IL-4 (50 ng/mL) produced large amounts of IL-12p70, low IL-10 and primed naïve CD4+ T cells to become Th1 cells. Thus, we demonstrate that the Th2 cell cytokine IL-4 decisively determines the phenotype of ongoing immune responses by orchestrating the functional phenotype of newly immigrating DC precursors.
Keywords
Dendritic Cells/immunology, Dose-Response Relationship, Drug, Humans, Immunophenotyping, Interferon-gamma/biosynthesis, Interleukin-10/biosynthesis, Interleukin-12/biosynthesis, Interleukin-4/pharmacology, Th1 Cells/immunology, Time Factors
Pubmed
Web of science
Create date
27/08/2020 15:00
Last modification date
30/09/2020 6:27
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