IL-4-mediated fine tuning of IL-12p70 production by human DC.
Details
Serval ID
serval:BIB_6E6909AF9F13
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
IL-4-mediated fine tuning of IL-12p70 production by human DC.
Journal
European journal of immunology
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Publication state
Published
Issued date
11/2008
Peer-reviewed
Oui
Volume
38
Number
11
Pages
3138-3149
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
IL-4 is expressed at high levels in allergic diseases and dominates the early phases of multiple acquired immune responses. However, the precise role of IL-4 during early inflammation and its impact on the differentiation of newly recruited DC precursors remains elusive. In order to characterize the impact of IL-4 on the differentiation of human DC, we investigated the role of IL-4 on the differentiation of monocytes into DC. Human DC were differentiated from peripheral blood precursors under either low or high concentrations of IL-4. We analyzed their cytokine profile and capacity to polarize T-cell differentiation. Concentrations of 5 (low) and 50 (high) ng/mL IL-4 induced two distinct types of DC. DC differentiated under low-dose IL-4 (5 ng/mL) produced almost no IL-12p70, and primed naïve CD4+ T cells allowing IL-4 secretion and Th2 induction. In contrast, DC generated under high concentrations of IL-4 (50 ng/mL) produced large amounts of IL-12p70, low IL-10 and primed naïve CD4+ T cells to become Th1 cells. Thus, we demonstrate that the Th2 cell cytokine IL-4 decisively determines the phenotype of ongoing immune responses by orchestrating the functional phenotype of newly immigrating DC precursors.
Keywords
Dendritic Cells/immunology, Dose-Response Relationship, Drug, Humans, Immunophenotyping, Interferon-gamma/biosynthesis, Interleukin-10/biosynthesis, Interleukin-12/biosynthesis, Interleukin-4/pharmacology, Th1 Cells/immunology, Time Factors
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Create date
27/08/2020 14:00
Last modification date
18/05/2022 5:36