Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis
Details
Serval ID
serval:BIB_6E509B10BF97
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Altered peptide ligands trigger perforin- rather than Fas-dependent cell lysis
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
11/1997
Volume
159
Number
9
Pages
4165-70
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 1
Research Support, Non-U.S. Gov't --- Old month value: Nov 1
Abstract
CTLs lyse Fas-expressing target cells by the concomitant action of a perforin- and a Fas-dependent mechanism. This study analyzed whether target cells pulsed with T cell antagonists and other altered peptide ligands (APLs) were susceptible selectively to only one of these two mechanisms. In vivo and in vitro activated T cells from transgenic mice expressing a TCR specific for lymphocytic choriomeningitis virus were used as effector cells. To distinguish between perforin- and Fas-dependent cytotoxicity, T cells from normal or perforin-deficient mice were used to lyse peptide-pulsed Fas-positive or Fas-negative target cells. In contrast to previous reports that have shown that APLs selectively induce the Fas-dependent pathway of cytotoxicity, our results demonstrate that target cells pulsed with T cell antagonists and other APLs are lysed predominantly by the perforin-dependent pathway. The contribution of Fas-mediated cytotoxicity was similar for the full agonist and the APLs. Thus, full agonists, partial agonists, and antagonists trigger similar and not distinct pathways of cytotoxicity.
Keywords
Animals
Antigens, CD95/*immunology
Cell Death/immunology
*Cytotoxicity, Immunologic
Fas Ligand Protein
Membrane Glycoproteins/*immunology
Mice
Mice, Transgenic
Pore Forming Cytotoxic Proteins
Receptors, Antigen, T-Cell/genetics/*immunology
T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Create date
28/01/2008 12:32
Last modification date
20/08/2019 15:27