Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

Ninet,  B.; Jan,  I.; Bontems,  O.; Lechenne,  B.; Jousson,  O.; Panizzon,  R.; Lew,  D.; Monod,  M.

doi:10.1128/JCM.41.2.826-830.2003

Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

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Serval ID

serval:BIB_6E041C84545D

Type

Article: article from journal or magazin.

Collection

Publications

Institution

UNIL/CHUV

Title

Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

Journal

Journal of Clinical Microbiology

Author(s)

Ninet B., Jan I., Bontems O., Lechenne B., Jousson O., Panizzon R., Lew D., Monod M.

ISSN

0095-1137 (Print)

Publication state

Published

Issued date

02/2003

Volume

Number

Pages

826-30

Notes

Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb

Abstract

We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.

Keywords

Animals Arthrodermataceae/*isolation & purification Base Sequence Cats DNA, Fungal/analysis Dogs Humans Molecular Sequence Data Polymerase Chain Reaction RNA, Ribosomal, 28S/*analysis/genetics Reagent Kits, Diagnostic Sequence Homology, Nucleic Acid

OAI-PMH

oai:serval.unil.ch:BIB_6E041C84545D

DOI

10.1128/JCM.41.2.826-830.2003

Pubmed

12574293

Web of science

000180891200053

Open Access

Yes

Create date

25/01/2008 16:54