Targeting of DNA polymerase to the adenovirus origin of DNA replication by interaction with nuclear factor I.

Details

Serval ID
serval:BIB_6DFE18C8C6DE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Targeting of DNA polymerase to the adenovirus origin of DNA replication by interaction with nuclear factor I.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Armentero M.T., Horwitz M., Mermod N.
ISSN
0027-8424[print], 0027-8424[linking]
Publication state
Published
Issued date
11/1994
Volume
91
Number
24
Pages
11537-11541
Language
english
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Efficient initiation by the DNA polymerase of adenovirus type 2 requires nuclear factor I (NFI), a cellular sequence-specific transcription factor. Three functions of NFI--dimerization, DNA binding, and activation of DNA replication--are colocalized within the N-terminal portion of the protein. To define more precisely the role of NFI in viral DNA replication, a series of site-directed mutations within the N-terminal domain have been generated, thus allowing the separation of all three functions contained within this region. Impairment of the dimerization function prevents sequence-specific DNA binding and in turn abolishes the NFI-mediated activation of DNA replication. NFI DNA-binding activity, although necessary, is not sufficient to activate the initiation of adenovirus replication. A distinct class of NFI mutations that abolish the recruitment of the viral DNA polymerase to the origin also prevent the activation of replication. Thus, a direct interaction of NFI with the viral DNA polymerase complex is required to form a stable and active preinitiation complex on the origin and is responsible for the activation of replication by NFI.
Keywords
Adenoviruses, Human/genetics, Amino Acid Sequence, Base Sequence, DNA Primers/chemistry, DNA Replication, DNA-Binding Proteins/metabolism, DNA-Directed DNA Polymerase/metabolism, Molecular Sequence Data, Mutagenesis, Site-Directed, NFI Transcription Factors, Protein Binding, Structure-Activity Relationship, Transcription Factors/metabolism, Viral Proteins/metabolism, Virus Replication
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 10:41
Last modification date
20/08/2019 14:27
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