Genes involved in the astrocyte-neuron lactate shuttle (ANLS) are specifically regulated in cortical astrocytes following sleep deprivation in mice.

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State: Public
Version: author
Serval ID
serval:BIB_6DFA76BF3602
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genes involved in the astrocyte-neuron lactate shuttle (ANLS) are specifically regulated in cortical astrocytes following sleep deprivation in mice.
Journal
Sleep
Author(s)
Petit J.M., Gyger J., Burlet-Godinot S., Fiumelli H., Martin J.L., Magistretti P.J.
ISSN
1550-9109 (Electronic)
ISSN-L
0161-8105
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
36
Number
10
Pages
1445-1458
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Abstract
STUDY OBJECTIVES: There is growing evidence indicating that in order to meet the neuronal energy demands, astrocytes provide lactate as an energy substrate for neurons through a mechanism called "astrocyte-neuron lactate shuttle" (ANLS). Since neuronal activity changes dramatically during vigilance states, we hypothesized that the ANLS may be regulated during the sleep-wake cycle. To test this hypothesis we investigated the expression of genes associated with the ANLS specifically in astrocytes following sleep deprivation. Astrocytes were purified by fluorescence-activated cell sorting from transgenic mice expressing the green fluorescent protein (GFP) under the control of the human astrocytic GFAP-promoter.
DESIGN: 6-hour instrumental sleep deprivation (TSD).
SETTING: Animal sleep research laboratory.
PARTICIPANTS: Young (P23-P27) FVB/N-Tg (GFAP-GFP) 14Mes/J (Tg) mice of both sexes and 7-8 week male Tg and FVB/Nj mice.
INTERVENTIONS: Basal sleep recordings and sleep deprivation achieved using a modified cage where animals were gently forced to move.
MEASUREMENTS AND RESULTS: Since Tg and FVB/Nj mice displayed a similar sleep-wake pattern, we performed a TSD in young Tg mice. Total RNA was extracted from the GFP-positive and GFP-negative cells sorted from cerebral cortex. Quantitative RT-PCR analysis showed that levels of Glut1, α-2-Na/K pump, Glt1, and Ldha mRNAs were significantly increased following TSD in GFP-positive cells. In GFP-negative cells, a tendency to increase, although not significant, was observed for Ldha, Mct2, and α-3-Na/K pump mRNAs.
CONCLUSIONS: This study shows that TSD induces the expression of genes associated with ANLS specifically in astrocytes, underlying the important role of astrocytes in the maintenance of the neuro-metabolic coupling across the sleep-wake cycle.
Keywords
Animals, Astrocytes/metabolism, Astrocytes/physiology, Electroencephalography, Electromyography, Female, Gene Expression Regulation/genetics, Gene Expression Regulation/physiology, Genes/physiology, Green Fluorescent Proteins/diagnostic use, Lactates/metabolism, Male, Mice, Mice, Transgenic, Motor Activity, Sleep/genetics, Sleep/physiology, Sleep Deprivation/genetics, Sleep Deprivation/physiopathology, Wakefulness/genetics, Wakefulness/physiology
Pubmed
Web of science
Open Access
Yes
Create date
06/08/2014 20:08
Last modification date
09/09/2019 6:26
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