Probing the T-cell receptor repertoire with deep sequencing.
Details
Serval ID
serval:BIB_6DB680DC3B78
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Probing the T-cell receptor repertoire with deep sequencing.
Journal
Current Opinion In Hiv and Aids
ISSN
1746-6318 (Electronic)
ISSN-L
1746-630X
Publication state
Published
Issued date
2012
Volume
7
Number
1
Pages
64-70
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; ReviewPublication Status: ppublish
Abstract
PURPOSE OF REVIEW: To review major findings on the T-cell receptor (TCR) repertoire diversity in response to several viral infections based on conventional methods of PCR, cloning and sequencing and to discuss their limitations in light of the recent methodological advances in deep sequencing.¦RECENT FINDINGS: Direct sequencing of TCR expressed by Ag-specific T cells isolated ex vivo has revealed that the TCR repertoire is not as restricted as previously estimated. Furthermore, analyses performed independently of the T-cell clonal hierarchy have brought to light an unexpected diversity. The choice of methods is critical to characterize the complexity of the repertoire. Recent advances in deep sequencing have uncovered the diversity of the TCR repertoire and shown that the size of the repertoire in naive and Ag-experienced memory T cells is three-fold to 15-fold larger than formerly estimated. Interestingly, the TCR complementary determining region 3 sequences are not randomly selected and a certain degree of shared TCR repertoire has been observed between different individuals.¦SUMMARY: Deep sequencing is a major methodological advance allowing more accurate molecular characterization of the TCR repertoire. In the near future, such technologies will further contribute to delineate the complexity of pathogen-specific T-cell response and help defining correlates of a protective immunity.
Keywords
Antigens, Viral/immunology, Cells, Cultured, High-Throughput Nucleotide Sequencing/methods, Humans, Polymorphism, Genetic, Receptors, Antigen, T-Cell/genetics, Receptors, Antigen, T-Cell/immunology, Virus Diseases/immunology
Pubmed
Web of science
Create date
06/06/2012 19:12
Last modification date
20/08/2019 15:27