Binding of Lassa virus perturbs extracellular matrix-induced signal transduction via dystroglycan.

Details

Ressource 1Request a copy Sous embargo indéterminé.
State: Public
Version: author
Secondary document(s)
Download: 22405130_Postprint.pdf (4102.30 [Ko])
State: Public
Version: author
Serval ID
serval:BIB_6D5EB21194EB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Binding of Lassa virus perturbs extracellular matrix-induced signal transduction via dystroglycan.
Journal
Cellular Microbiology
Author(s)
Rojek J.M., Moraz M.L., Pythoud C., Rothenberger S., Van der Goot F.G., Campbell K.P., Kunz S.
ISSN
1462-5822 (Electronic)
ISSN-L
1462-5814
Publication state
Published
Issued date
2012
Volume
14
Number
7
Pages
1122-1134
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The arenavirus Lassa virus (LASV) causes a severe haemorrhagic fever with high mortality in man. The cellular receptor for LASV is dystroglycan (DG). DG is a ubiquitous receptor for extracellular matrix (ECM) proteins, which cooperates with β1 integrins to control cell-matrix interactions. Here, we investigated whether LASV binding to DG triggers signal transduction, mimicking the natural ligands. Engagement of DG by LASV resulted in the recruitment of the adaptor protein Grb2 and the protein kinase MEK1 by the cytoplasmic domain of DG without activating the MEK/ERK pathway, indicating assembly of an inactive signalling complex. LASV binding to cells however affected the activation of the MEK/ERK pathway via α6β1 integrins. The virus-induced perturbation of α6β1 integrin signalling critically depended on high-affinity LASV binding to DG and DG's cytoplasmic domain, indicating that LASV-receptor binding perturbed signalling cross-talk between DG and β1 integrins.
Keywords
Antigens, CD29/metabolism, Cell Line, Dystroglycans/metabolism, Extracellular Matrix/metabolism, Humans, Lassa virus/pathogenicity, Lassa virus/physiology, Models, Biological, Receptors, Virus/metabolism, Signal Transduction, Virus Attachment
Pubmed
Web of science
Create date
22/07/2012 20:58
Last modification date
20/08/2019 14:27
Usage data