How to: identify non-tuberculous Mycobacterium species using MALDI-TOF mass spectrometry.

Details

Serval ID
serval:BIB_6D3B229D6392
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
How to: identify non-tuberculous Mycobacterium species using MALDI-TOF mass spectrometry.
Journal
Clinical microbiology and infection
Author(s)
Alcaide F., Amlerová J., Bou G., Ceyssens P.J., Coll P., Corcoran D., Fangous M.S., González-Álvarez I., Gorton R., Greub G., Hery-Arnaud G., Hrábak J., Ingebretsen A., Lucey B., Marekoviċ I., Mediavilla-Gradolph C., Monté M.R., O'Connor J., O'Mahony J., Opota O., O'Reilly B., Orth-Höller D., Oviaño M., Palacios J.J., Palop B., Pranada A.B., Quiroga L., Rodríguez-Temporal D., Ruiz-Serrano M.J., Tudó G., Van den Bossche A., van Ingen J., Rodriguez-Sanchez B.
Working group(s)
European Study Group on Genomics and Molecular Diagnosis (ESGMD)
ISSN
1469-0691 (Electronic)
ISSN-L
1198-743X
Publication state
Published
Issued date
06/2018
Peer-reviewed
Oui
Volume
24
Number
6
Pages
599-603
Language
english
Notes
Publication types: Journal Article ; Review
Publication Status: ppublish
Abstract
The implementation of MALDI-TOF MS for microorganism identification has changed the routine of the microbiology laboratories as we knew it. Most microorganisms can now be reliably identified within minutes using this inexpensive, user-friendly methodology. However, its application in the identification of mycobacteria isolates has been hampered by the structure of their cell wall. Improvements in the sample processing method and in the available database have proved key factors for the rapid and reliable identification of non-tuberculous mycobacteria isolates using MALDI-TOF MS.
The main objective is to provide information about the proceedings for the identification of non-tuberculous isolates using MALDI-TOF MS and to review different sample processing methods, available databases, and the interpretation of the results.
Results from relevant studies on the use of the available MALDI-TOF MS instruments, the implementation of innovative sample processing methods, or the implementation of improved databases are discussed.
Insight about the methodology required for reliable identification of non-tuberculous mycobacteria and its implementation in the microbiology laboratory routine is provided.
Microbiology laboratories where MALDI-TOF MS is available can benefit from its capacity to identify most clinically interesting non-tuberculous mycobacteria in a rapid, reliable, and inexpensive manner.
Keywords
Bacteriological Techniques, Humans, Mycobacterium Infections, Nontuberculous/diagnosis, Nontuberculous Mycobacteria/isolation & purification, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, Workflow, Identification, MALDI-TOF MS, Non-tuberculous mycobacteria, Procedure, Sample preparation
Pubmed
Web of science
Create date
30/11/2017 20:09
Last modification date
20/08/2019 15:26
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