No evidence for protein modifications in fresh frozen plasma after photochemical treatment: an analysis by high-resolution two-dimensional electrophoresis

Details

Serval ID
serval:BIB_6D30A5E5CCE2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
No evidence for protein modifications in fresh frozen plasma after photochemical treatment: an analysis by high-resolution two-dimensional electrophoresis
Journal
British Journal of Haematology
Author(s)
Tissot  J. D., Hochstrasser  D. F., Schneider  B., Morgenthaler  J. J., Schneider  P.
ISSN
0007-1048 (Print)
Publication state
Published
Issued date
01/1994
Volume
86
Number
1
Pages
143-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Abstract
To study if photochemical treatment of human plasma by methylene blue in combination with visible light induces protein alterations, we analysed by high-resolution two-dimensional gel electrophoresis (2-D PAGE) untreated and photochemically treated fresh frozen plasma collected by apheresis from the same donor (n = 8). Polypeptides were separated according to their charges by isoelectric focusing and to their sizes in polyacrylamide gels in presence of sodium and to their sizes in polyacrylamide gels in presence of sodium dodecyl sulphate, and visualized by silver staining. Despite their apparent complexity, protein maps of untreated plasma and photochemically treated fresh frozen plasma revealed identical spot patterns. Thus, photochemical treatment did not induce apparent protein pattern modifications.
Keywords
Blood Proteins/*drug effects Electrophoresis, Gel, Two-Dimensional Humans Isoelectric Focusing Methylene Blue/*pharmacology *Photochemotherapy Protein Denaturation/drug effects
Pubmed
Web of science
Create date
25/01/2008 15:34
Last modification date
20/08/2019 14:26
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