Pseudomonads as antagonists of plant pathogens in the rhizosphere: role of the antibiotic 2,4-diacetylphloroglucinol in the suppression of black root rot of tobacco
Details
Serval ID
serval:BIB_6D2A9C549DE7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Pseudomonads as antagonists of plant pathogens in the rhizosphere: role of the antibiotic 2,4-diacetylphloroglucinol in the suppression of black root rot of tobacco
Journal
Symbiosis
ISSN
0334-5114
Publication state
Published
Issued date
1990
Volume
9
Number
1-3
Pages
327-341
Language
english
Abstract
Pseudomonas fluorescens strain CHA0 is an effective biocontrol agent of diseases caused by soilborne plant pathogens. Strain CHA0 produces several secondary metabolites, notably cyanide, acetylpheoroglucinols and pyoluteorin. Cyanide plays an important role in the suppression of black root rot of tobacco, caused by Thielaviopsis basicola. A mutant, CHA625, has now been isolated, which does not produce 2,4-diacetylphloroglucinol. Strain CHA625 suppressed black root rot to a distinctly smaller extent than did wild-type CHA0 under gnotobiotic conditions. A cosmid obtained from a genomic library of strain CHA0 restored the ability of strain CHA625 to produce this metabolite and to suppress disease. Addition of synthetic 2,4-diacetylphloroglucinol to soil reduced disease severity in the absence of bacteria. These results suggest that the production of 2,4-diacetylphloroglucinol by P. fluorescens strain CHA0 is another important factor, in addition to cyanide, in the suppression of black root rot of tobacco.
Keywords
BIOCONTROL, SOILBORNE PATHOGENS, PSEUDOMONAS, ANTIBIOTICS, PHLOROGLUCINOL, CYANIDE
Web of science
Create date
17/02/2008 12:09
Last modification date
20/08/2019 15:26