Discovery and Characterization of Mycobacterium basiliense sp. nov., a Nontuberculous Mycobacterium Isolated From Human Lungs.

Details

Serval ID
serval:BIB_6CCF717EE6E2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Discovery and Characterization of Mycobacterium basiliense sp. nov., a Nontuberculous Mycobacterium Isolated From Human Lungs.
Journal
Frontiers in microbiology
Author(s)
Seth-Smith HMB, Imkamp F., Tagini F., Cuénod A., Hömke R., Jahn K., Tschacher A., Grendelmeier P., Bättig V., Erb S., Reinhard M., Rütimann G., Borrell S., Gagneux S., Casanova C., Droz S., Osthoff M., Tamm M., Nübel U., Greub G., Keller P.M., Egli A.
ISSN
1664-302X (Print)
ISSN-L
1664-302X
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
9
Pages
3184
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Bacteria belonging to the genus Mycobacterium are predominantly responsible for pulmonary diseases; most notably Mycobacterium tuberculosis causes granulomatous pulmonary infections. Here we describe a novel slow growing mycobacterial species isolated from respiratory samples from five patients, four with underlying pulmonary disease. The isolates were characterized by biochemical and molecular techniques, including whole genome sequencing. Biochemical characteristics generally match those of M. marinum and M. ulcerans; however, the most striking difference of the new species is its ability to grow at 37°C. The new species was found to grow in human macrophages, but not amoebae, suggesting a pathogenic rather than an environmental lifestyle. Phylogenetic analysis reveals a deep-rooting relationship to M. marinum and M. ulcerans. A complete genome sequence was obtained through combining short and long-read sequencing, providing a genome of 5.6 Mb. The genome appears to be highly intact, syntenic with that of M. marinum, with very few insertion sequences. A vast array of virulence factors includes 283 PE/PPE surface-associated proteins, making up 10% of the coding capacity, and 22 non-ribosomal peptide synthase clusters. A comparison of six clinical isolates from the five patients shows that they differ by up to two single nucleotide polymorphisms, suggesting a common source of infection. Our findings are in accordance with the recognition of a new taxonomic entity. We propose the name M. basiliense, as all isolates were found in patients from the Basel area of Switzerland.
Keywords
Microbiology (medical), Microbiology, Mycobacterium basiliense, nontuberculous mycobacteria, novel species, pathogen, virulence
Pubmed
Web of science
Open Access
Yes
Create date
20/01/2019 15:06
Last modification date
20/08/2019 14:26
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