Tuned SMC Arms Drive Chromosomal Loading of Prokaryotic Condensin.

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State: Public
Version: Final published version
Serval ID
serval:BIB_6CA09AC28052
Type
Article: article from journal or magazin.
Collection
Publications
Title
Tuned SMC Arms Drive Chromosomal Loading of Prokaryotic Condensin.
Journal
Molecular Cell
Author(s)
Bürmann F., Basfeld A., Vazquez Nunez R., Diebold-Durand M.L., Wilhelm L., Gruber S.
ISSN
1097-4164 (Electronic)
ISSN-L
1097-2765
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
65
Number
5
Pages
861-872.e9
Language
english
Abstract
SMC proteins support vital cellular processes in all domains of life by organizing chromosomal DNA. They are composed of ATPase "head" and "hinge" dimerization domains and a connecting coiled-coil "arm." Binding to a kleisin subunit creates a closed tripartite ring, whose ∼47-nm-long SMC arms act as barrier for DNA entrapment. Here, we uncover another, more active function of the bacterial Smc arm. Using high-throughput genetic engineering, we resized the arm in the range of 6-60 nm and found that it was functional only in specific length regimes following a periodic pattern. Natural SMC sequences reflect these length constraints. Mutants with improper arm length or peptide insertions in the arm efficiently target chromosomal loading sites and hydrolyze ATP but fail to use ATP hydrolysis for relocation onto flanking DNA. We propose that SMC arms implement force transmission upon nucleotide hydrolysis to mediate DNA capture or loop extrusion.

Pubmed
Web of science
Open Access
Yes
Create date
03/03/2017 11:31
Last modification date
20/08/2019 15:26
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