Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro
Details
Serval ID
serval:BIB_6C66E413D163
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro
Journal
Experimental Neurology
ISSN
0014-4886 (Print)
Publication state
Published
Issued date
2007
Volume
207
Number
2
Pages
267-274
Notes
DA - 20070925
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Glial Fibrillary Acidic Protein)
RN - 0 (Myelin Proteins)
RN - 0 (Nerve Tissue Proteins)
RN - 0 (Neuregulin-1)
SB - IM
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Glial Fibrillary Acidic Protein)
RN - 0 (Myelin Proteins)
RN - 0 (Nerve Tissue Proteins)
RN - 0 (Neuregulin-1)
SB - IM
Abstract
Experimentally, peripheral nerve repair can be enhanced by Schwann cell transplantation but the clinical application is limited by donor site morbidity and the inability to generate a sufficient number of cells quickly. We have investigated whether adult stem cells, isolated from adipose tissue, can be differentiated into functional Schwann cells. Rat visceral fat was enzymatically digested to yield rapidly proliferating fibroblast-like cells, a proportion of which expressed the mesenchymal stem cell marker, stro-1, and nestin, a neural progenitor protein. Cells treated with a mixture of glial growth factors (GGF-2, bFGF, PDGF and forskolin) adopted a spindle-like morphology similar to Schwann cells. Immunocytochemical staining and western blotting indicated that the treated cells expressed the glial markers, GFAP, S100 and p75, indicative of differentiation. When co-cultured with NG108-15 motor neuron-like cells, the differentiated stem cells enhanced the number of NG108-15 cells expressing neurites, the number of neurites per cell and the mean length of the longest neurite extended. Schwann cells evoked a similar response whilst undifferentiated stem cells had no effect. These results indicate adipose tissue contains a pool of regenerative stem cells which can be differentiated to a Schwann cell phenotype and may be of benefit for treatment of peripheral nerve injuries
Keywords
Adipose Tissue/Adult Stem Cells/Animals/Cell Count/Cell Differentiation/Cell Proliferation/Coculture Techniques/cytology/drug effects/Glial Fibrillary Acidic Protein/metabolism/methods/Myelin Proteins/Nerve Tissue Proteins/Neuregulin-1/Neurites/Neuroblastoma/Neurons/pathology/pharmacology/physiology/Rats/Rats,Sprague-Dawley/Schwann Cells/Time Factors
Pubmed
Web of science
Create date
18/02/2008 10:27
Last modification date
20/08/2019 15:26