Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani.

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State: Public
Version: Final published version
Serval ID
serval:BIB_6C624F1425F6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani.
Journal
Infection and immunity
Author(s)
McFarlane E., Perez C., Charmoy M., Allenbach C., Carter K.C., Alexander J., Tacchini-Cottier F.
ISSN
1098-5522[electronic]
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
76
Number
2
Pages
532-541
Language
english
Abstract
Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The role of neutrophils during the first weeks post-infection with Leishmania donovani was investigated in this study. When L. donovani-infected mice were selectively depleted of neutrophils with the NIMP-R14 monoclonal antibody, a significant increase in parasite numbers was observed in the spleen and bone marrow and to a lesser extent in the liver. Increased susceptibility was associated with enhanced splenomegally, a delay in the maturation of hepatic granulomas, and a decrease in inducible nitric oxide synthase expression within granulomas. In the spleen, neutrophil depletion was associated with a significant increase in interleukin 4 (IL-4) and IL-10 levels and reduced gamma interferon secretion by CD4(+) and CD8(+) T cells. Increased production of serum IL-4 and IL-10 and higher levels of Leishmania-specific immunoglobulin G1 (IgG1) versus IgG2a revealed the preferential induction of Th2 responses in neutrophil-depleted mice. Altogether, these data suggest a critical role for neutrophils in the early protective response against L. donovani, both as effector cells involved in the killing of the parasites and as significant players influencing the development of a protective Th1 immune response.
Keywords
Animals, Bone Marrow/parasitology, CD4-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/immunology, Female, Granuloma/pathology, Interferon-gamma/metabolism, Interleukin-10/analysis, Interleukin-4/analysis, Leishmania donovani/immunology, Leishmaniasis, Visceral/immunology, Leukocyte Reduction Procedures, Liver/parasitology, Liver/pathology, Mice, Neutrophils/immunology, Nitric Oxide Synthase/biosynthesis, Spleen/chemistry, Spleen/parasitology, Splenomegaly/parasitology, Th1 Cells/immunology
Pubmed
Web of science
Open Access
Yes
Create date
17/08/2009 14:18
Last modification date
20/08/2019 14:26
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