The potential of microfluidic water-in-oil droplets in experimental biology.
Details
Serval ID
serval:BIB_6C063C2047E8
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
The potential of microfluidic water-in-oil droplets in experimental biology.
Journal
Molecular bioSystems
ISSN
1742-2051 (Electronic)
ISSN-L
1742-2051
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
5
Number
12
Pages
1392-1404
Language
english
Abstract
The comprehensive characterisation of complex parameter space in '-omics' technologies requires high-throughput systems. In vitro compartmentalisation of reactions in water-in-oil droplets combines the necessary ability to carry out large numbers of experiments under controlled conditions with quantitative readout, and has recently advanced towards automation by generating droplets in microfluidic devices. Some approaches based on these principles are already familiar (e.g. emulsion PCR for sequencing), others, including directed evolution or cell-based assays, are in advanced stages of development--and proof-of-principle experiments are appearing for a whole range of applications in diagnostics, cellomics, proteomics, drug discovery and systems and synthetic biology. This review describes the current state-of-the-art, notes salient features of successful experiments and extrapolates in the direction of more highly integrated systems.
Keywords
Computational Biology/methods, Directed Molecular Evolution/methods, Drug Discovery/methods, Emulsions, Microfluidic Analytical Techniques/instrumentation, Microfluidic Analytical Techniques/methods, Oils/chemistry, Polymerase Chain Reaction/methods, Water/chemistry
Pubmed
Web of science
Create date
02/02/2017 9:13
Last modification date
20/08/2019 15:26