The CpG-rich promoter of human LDH-C is differentially methylated in expressing and nonexpressing tissues

Details

Serval ID
serval:BIB_6BDC6F769238
Type
Article: article from journal or magazin.
Collection
Publications
Title
The CpG-rich promoter of human LDH-C is differentially methylated in expressing and nonexpressing tissues
Journal
Developmental Genetics
Author(s)
Bonny  C., Goldberg  E.
ISSN
0192-253X (Print)
Publication state
Published
Issued date
1995
Volume
16
Number
2
Pages
210-7
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
Abstract
A comparison of nucleotide sequences of murine Ldh-a and Ldh-c genes and human LDH-A, LDH-B, and LDH-C reveals that mouse Ldh-c has lost the CpG "island" present in the genes for the somatic isozymes. However, the human LDH-C gene has a CpG-rich region of 230 bp surrounding its promoter. Endonuclease sensitivity coupled with polymerase chain reaction (PCR) demonstrate the presence of nine heavily methylated sites in this region in different somatic cells. The same sites are specifically hypomethylated in expressing tissues. 3' sites bordering the CpG-rich region appear to be methylated in both expressing and nonexpressing tissues. Furthermore, the methylated promoter forms a specific complex in vitro with a methyl-DNA binding protein. Evolutionary and functional implications of these observations are discussed.
Keywords
Base Sequence DNA/metabolism DNA Primers Dinucleoside Phosphates/*metabolism Endonucleases/metabolism Hela Cells Humans Isoenzymes L-Lactate Dehydrogenase/*genetics/metabolism Male Methylation Molecular Sequence Data *Promoter Regions (Genetics) Testis/metabolism
Pubmed
Web of science
Create date
25/01/2008 14:16
Last modification date
20/08/2019 14:26
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