Proteomic Characterization of the Venom of Five Bombus (Thoracobombus) Species.

Details

Serval ID
serval:BIB_6BD2EBEB2FC9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Proteomic Characterization of the Venom of Five Bombus (Thoracobombus) Species.
Journal
Toxins
Author(s)
Barkan N.P., Bayazit M.B., Ozel Demiralp D.
ISSN
2072-6651 (Electronic)
ISSN-L
2072-6651
Publication state
Published
Issued date
11/11/2017
Peer-reviewed
Oui
Volume
9
Number
11
Pages
NA
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Venomous animals use venom, a complex biofluid composed of unique mixtures of proteins and peptides, to act on vital systems of the prey or predator. In bees, venom is solely used for defense against predators. However, the venom composition of bumble bees ( <i>Bombus</i> sp.) is largely unknown. The <i>Thoracobombus</i> subgenus of <i>Bombus</i> sp. is a diverse subgenus represented by 14 members across Turkey. In this study, we sought out to proteomically characterize the venom of five <i>Thoracobombus</i> species by using bottom-up proteomic techniques. We have obtained two-dimensional polyacrylamide gel (2D-PAGE) images of each species' venom sample. We have subsequently identified the protein spots by using matrix assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS). We have identified 47 proteins for <i>Bombus humilis</i> , 32 for <i>B. pascuorum</i> , 60 for <i>B. ruderarius</i> , 39 for <i>B. sylvarum</i> , and 35 for <i>B. zonatus</i> . Moreover, we illustrated that intensities of 2DE protein spots corresponding to putative venom toxins vary in a species-specific manner. Our analyses provide the primary proteomic characterization of five bumble bee species' venom composition.
Keywords
Animals, Bee Venoms/metabolism, Bees/classification, Bees/metabolism, Electrophoresis, Gel, Two-Dimensional, Proteomics, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, 2D-PAGE, MALDI-TOF MS, bumble bees, proteomics, toxins, venom
Pubmed
Web of science
Open Access
Yes
Create date
16/11/2017 21:32
Last modification date
20/08/2019 15:26
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