Ricca's factors as mobile proteinaceous effectors of electrical signaling.

Details

Ressource 1Download: Ricca’s factors as mobile proteinaceous effectors of electrical signaling.pdf (9102.92 [Ko])
State: Public
Version: Final published version
License: CC BY-NC 4.0
Serval ID
serval:BIB_6BB3D51CA8D3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ricca's factors as mobile proteinaceous effectors of electrical signaling.
Journal
Cell
Author(s)
Gao Y.Q., Jimenez-Sandoval P., Tiwari S., Stolz S., Wang J., Glauser G., Santiago J., Farmer E.E.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Publication state
Published
Issued date
30/03/2023
Peer-reviewed
Oui
Volume
186
Number
7
Pages
1337-1351.e20
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Leaf-feeding insects trigger high-amplitude, defense-inducing electrical signals called slow wave potentials (SWPs). These signals are thought to be triggered by the long-distance transport of low molecular mass elicitors termed Ricca's factors. We sought mediators of leaf-to-leaf electrical signaling in Arabidopsis thaliana and identified them as β-THIOGLUCOSIDE GLUCOHYDROLASE 1 and 2 (TGG1 and TGG2). SWP propagation from insect feeding sites was strongly attenuated in tgg1 tgg2 mutants and wound-response cytosolic Ca <sup>2+</sup> increases were reduced in these plants. Recombinant TGG1 fed into the xylem elicited wild-type-like membrane depolarization and Ca <sup>2+</sup> transients. Moreover, TGGs catalyze the deglucosidation of glucosinolates. Metabolite profiling revealed rapid wound-induced breakdown of aliphatic glucosinolates in primary veins. Using in vivo chemical trapping, we found evidence for roles of short-lived aglycone intermediates generated by glucosinolate hydrolysis in SWP membrane depolarization. Our findings reveal a mechanism whereby organ-to-organ protein transport plays a major role in electrical signaling.
Keywords
Animals, Glycoside Hydrolases/metabolism, Glucosinolates/metabolism, Arabidopsis/metabolism, Arabidopsis Proteins/metabolism, Insecta, glucosinolate, herbivore, insect, jasmonate, membrane potential, myrosinase, slow wave potential, wound, xylem
Pubmed
Web of science
Open Access
Yes
Create date
13/03/2023 12:29
Last modification date
25/06/2024 7:31
Usage data